Nathan Line sandbox 1: Difference between revisions
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The peptide is pulled into the active site by Asp 189. His 57 pulls on the hydrogen attached to the OH on Ser 195 to make it a better nucleophilie which attacks the carbonyl of the peptide. This tetrahedral intermediate removes the hydrogen from His 57 while the carbonyl reforms resulting in a break in the peptide backbone. After the amino group is replaced with water, the water again attacks the carbonyl and this tetrahedral collapses to reconstruct both the Ser195 and His57. The final products result in an amino group and a carboxylic acid compound. | The peptide is pulled into the active site by Asp 189. His 57 pulls on the hydrogen attached to the OH on Ser 195 to make it a better nucleophilie which attacks the carbonyl of the peptide. This tetrahedral intermediate removes the hydrogen from His 57 while the carbonyl reforms resulting in a break in the peptide backbone. After the amino group is replaced with water, the water again attacks the carbonyl and this tetrahedral collapses to reconstruct both the Ser195 and His57. The final products result in an amino group and a carboxylic acid compound. | ||
{{STRUCTURE_2ah4 | PDB=2ah4 | SCENE= }} | |||
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Revision as of 17:44, 19 February 2010
The Mechanism of TrypsinThe Mechanism of Trypsin
The peptide is pulled into the active site by Asp 189. His 57 pulls on the hydrogen attached to the OH on Ser 195 to make it a better nucleophilie which attacks the carbonyl of the peptide. This tetrahedral intermediate removes the hydrogen from His 57 while the carbonyl reforms resulting in a break in the peptide backbone. After the amino group is replaced with water, the water again attacks the carbonyl and this tetrahedral collapses to reconstruct both the Ser195 and His57. The final products result in an amino group and a carboxylic acid compound.
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| 2ah4, resolution 1.13Å () | |||||||||
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| Ligands: | , , | ||||||||
| Activity: | Trypsin, with EC number 3.4.21.4 | ||||||||
| Related: | 2age, 2agg, 2agi | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
