Group:SMART:2010 Pingry SMART Team: Difference between revisions
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A key component of Dr. Banta’s work is engineering AdhD to accept a broad range of substrates. This is a crucial component of his work, because this enzyme will be required to act upon a wide range of substrates when it is used within a practical biofuel cell. Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase demonstrates how an enzyme is specific to certain substrates and therefore help show what might be done to broaden the specificity of an enzyme. The function of this enzyme within the rat liver is to regulate/ inactivate steroid hormones. The enzyme does this is by reducing the steroid’s (testosterone) C3 ketone group. The interactions within the active site and testosterone are very specific, because of the structure and positioning of the residues within the cavity. This information is important, because it will help show what might be done to adhd to broaden its substrate specificity. | A key component of Dr. Banta’s work is engineering AdhD to accept a broad range of substrates. This is a crucial component of his work, because this enzyme will be required to act upon a wide range of substrates when it is used within a practical biofuel cell. Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase demonstrates how an enzyme is specific to certain substrates and therefore help show what might be done to broaden the specificity of an enzyme. The function of this enzyme within the rat liver is to regulate/ inactivate steroid hormones. The enzyme does this is by reducing the steroid’s (testosterone) C3 ketone group. The interactions within the active site and testosterone are very specific, because of the structure and positioning of the residues within the cavity. This information is important, because it will help show what might be done to adhd to broaden its substrate specificity. | ||
<applet load='1LWI_chainA.pdb' size='400' frame='true' align='left' caption='1lwi, Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase with NADP+ cofactor' scene='2010_Pingry_SMART_Team/1lwi_default/6'/> | <applet load='1LWI_chainA.pdb' size='400' frame='true' align='left' caption='1lwi, Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase with NADP+ cofactor' scene='2010_Pingry_SMART_Team/1lwi_default/6'/> | ||
====PDB ID: 1lwi, Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase with NADP+ cofactor==== | ====PDB ID: 1lwi, Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase with NADP+ cofactor==== | ||
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<scene name='2010_Pingry_SMART_Team/1lwi_default/6'>Revert to default scene display</scene> | <scene name='2010_Pingry_SMART_Team/1lwi_default/6'>Revert to default scene display</scene> | ||
{{Clear}} | {{Clear}} | ||
<applet load='1AFS_chainA.pdb' size='400' frame='true' align='left' caption='1afs, Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase with cofactor and testosterone' scene='2010_Pingry_SMART_Team/1afs_default/5'/> | <applet load='1AFS_chainA.pdb' size='400' frame='true' align='left' caption='1afs, Rat liver 3-alpha-hydroxysteroid dihydrodiol dehydrogenase with cofactor and testosterone' scene='2010_Pingry_SMART_Team/1afs_default/5'/> | ||
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<scene name='2010_Pingry_SMART_Team/1afs_default/5'>Revert to default scene display</scene> | <scene name='2010_Pingry_SMART_Team/1afs_default/5'>Revert to default scene display</scene> | ||