Group:SMART:2010 Pingry SMART Team: Difference between revisions
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<scene name='2010_Pingry_SMART_Team/1a80-default/1'>The backbone of the four residues changed between WT and NADP-binding mutant are colored orange (Lys232, Phe22, Arg238, Ala272).</scene> | <scene name='2010_Pingry_SMART_Team/1a80-default/1'>The backbone of the four residues changed between WT and NADP-binding mutant are colored orange (Lys232, Phe22, Arg238, Ala272).</scene> | ||
Other residues highlighted by displaying sidechain: | Other residues highlighted by displaying sidechain: | ||
Trp187; pi-stacking interaction with the nicotinamide ring of the cofactor. | Trp187; pi-stacking interaction with the nicotinamide ring of the cofactor that stabilizes the reaction. | ||
Lys232, Ser233, Val234; interacts with phosphate group of NADPH. These are changed in the mutant form in order to accommodate for the cofactor NADH. | |||
Not shown: | Not shown: | ||