Group:SMART:2010 Pingry SMART Team: Difference between revisions
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==='''Inherent dual cofactor use, Xylose reductase'''=== | ==='''Inherent dual cofactor use, Xylose reductase'''=== | ||
<applet load='1K8C_chainD.pdb' size='400' frame='true' align='left' caption='1k8c, Xylose reductase with NADP+' scene='2010_Pingry_SMART_Team/1k8c_default/2'/> | <applet load='1K8C_chainD.pdb' size='400' frame='true' align='left' caption='1k8c, Xylose reductase with NADP+' scene='2010_Pingry_SMART_Team/1k8c_default/2'/> | ||
Pink and blue highlight the (alpha/beta)8 barrel structure of AKR's. | |||
Cofactor (NADP+) shown in wireframe and colored CPK. | |||
The residues Glu227, Lys274, Ser275, Asn276, Arg280 have sidechains shown in wireframe and colored green. | |||
Glu227-Changes its interactions with the cofactor depending upon if the cofactor is NAD or NADP, it has water-mediated reaction with the 3’ alcohol group on the ribose. | |||
Lys274-interacts with the 2’ alcohol group on the NADP ribose but does not interact with the NAD cofactor | |||
Ser275- interacts with an oxygen on the NADP ribose’s phosphate group but does not interact with the NAD cofactor | |||
Asn276-hydrogen bonding interactions with the different cofactors change when the cofactor changes | |||
Arg280-changes position and interacts differently with the two types of cofactors. | |||
====PDB ID: 1k8c, Xylose reductase with NADP+==== | ====PDB ID: 1k8c, Xylose reductase with NADP+==== | ||