1ymo: Difference between revisions
New page: left|200px<br /><applet load="1ymo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ymo" /> '''Solution structure of the P2b-P3 pseudoknot ... |
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[[Image:1ymo.gif|left|200px]]<br /><applet load="1ymo" size=" | [[Image:1ymo.gif|left|200px]]<br /><applet load="1ymo" size="350" color="white" frame="true" align="right" spinBox="true" | ||
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'''Solution structure of the P2b-P3 pseudoknot from human telomerase RNA'''<br /> | '''Solution structure of the P2b-P3 pseudoknot from human telomerase RNA'''<br /> | ||
==Overview== | ==Overview== | ||
Human telomerase contains a 451 nt RNA (hTR) and several proteins, including a specialized reverse transcriptase (hTERT). The 5' half of hTR | Human telomerase contains a 451 nt RNA (hTR) and several proteins, including a specialized reverse transcriptase (hTERT). The 5' half of hTR comprises the pseudoknot (core) domain, which includes the RNA template for telomere synthesis and a highly conserved pseudoknot that is required for telomerase activity. The solution structure of this essential pseudoknot, presented here, reveals an extended triple helix surrounding the helical junction. The network of tertiary interactions explains the phylogenetic sequence conservation and existing human and mouse TR functional studies as well as mutations linked to disease. Thermodynamic stability, dimerization potential, and telomerase activity of mutant RNAs that alter the tertiary contacts were investigated. Telomerase activity is strongly correlated with tertiary structure stability, whereas there is no correlation with dimerization potential of the pseudoknot. These studies reveal that a conserved pseudoknot tertiary structure is required for telomerase activity. | ||
==About this Structure== | ==About this Structure== | ||
1YMO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | 1YMO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YMO OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the human telomerase RNA pseudoknot reveals conserved tertiary interactions essential for function., Theimer CA, Blois CA, Feigon J, Mol Cell. 2005 Mar 4;17(5):671-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15749017 15749017] | Structure of the human telomerase RNA pseudoknot reveals conserved tertiary interactions essential for function., Theimer CA, Blois CA, Feigon J, Mol Cell. 2005 Mar 4;17(5):671-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15749017 15749017] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Blois, C | [[Category: Blois, C A.]] | ||
[[Category: Feigon, J.]] | [[Category: Feigon, J.]] | ||
[[Category: Theimer, C | [[Category: Theimer, C A.]] | ||
[[Category: hoogsteen]] | [[Category: hoogsteen]] | ||
[[Category: non-canonical]] | [[Category: non-canonical]] | ||
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[[Category: triplex]] | [[Category: triplex]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:06:56 2008'' | ||
