1qhv: Difference between revisions

Revision as of 15:39, 21 February 2008

HUMAN ADENOVIRUS SEROTYPE 2 FIBRE HEAD

OverviewOverview

Adenovirus binds to its receptor via the head domain of its fiber protein. We have crystallized the adenovirus serotype 2 (subgroup C) receptor binding domain and solved the structure at 1.5 A resolution by the molecular replacement technique using the known adenovirus type 5 head structure. Included in the high-resolution model are 306 water molecules, five alternative side chain conformations, and individual anisotropic temperature factors for each atom. The overall structure of the serotype 2 head is very similar to its serotype 5 homologue, apart from differences in some of the flexible loops. All but subgroup B adenoviruses are believed to use the recently identified protein CAR (Coxsackievirus and adenovirus receptor) as receptor. By comparison of the two structures and sequence alignment of CAR binding and non-CAR binding serotype fiber heads, we discuss possible receptor binding sites and propose a receptor binding site in a crevice between two monomers on the side of the trimer. The structural basis of the extraordinary stability of the fiber head trimer is also discussed.

About this StructureAbout this Structure

1QHV is a Single protein structure of sequence from Human adenovirus 2 with as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the human adenovirus serotype 2 fiber head domain at 1.5 A resolution., van Raaij MJ, Louis N, Chroboczek J, Cusack S, Virology. 1999 Sep 30;262(2):333-43. PMID:10502512

Page seeded by OCA on Thu Feb 21 14:39:44 2008

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OCA

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-[[Image:1qhv.jpg|left|200px]]<br /><applet load="1qhv" size="450" color="white" frame="true" align="right" spinBox="true"
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 caption="1qhv, resolution 1.51&Aring;" />
 '''HUMAN ADENOVIRUS SEROTYPE 2 FIBRE HEAD'''<br />
 ==Overview==
-Adenovirus binds to its receptor via the head domain of its fiber protein., We have crystallized the adenovirus serotype 2 (subgroup C) receptor, binding domain and solved the structure at 1.5 A resolution by the, molecular replacement technique using the known adenovirus type 5 head, structure. Included in the high-resolution model are 306 water molecules, five alternative side chain conformations, and individual anisotropic, temperature factors for each atom. The overall structure of the serotype 2, head is very similar to its serotype 5 homologue, apart from differences, in some of the flexible loops. All but subgroup B adenoviruses are, believed to use the recently identified protein CAR (Coxsackievirus and, adenovirus receptor) as receptor. By comparison of the two structures and, sequence alignment of CAR binding and non-CAR binding serotype fiber, heads, we discuss possible receptor binding sites and propose a receptor, binding site in a crevice between two monomers on the side of the trimer., The structural basis of the extraordinary stability of the fiber head, trimer is also discussed.
+Adenovirus binds to its receptor via the head domain of its fiber protein. We have crystallized the adenovirus serotype 2 (subgroup C) receptor binding domain and solved the structure at 1.5 A resolution by the molecular replacement technique using the known adenovirus type 5 head structure. Included in the high-resolution model are 306 water molecules, five alternative side chain conformations, and individual anisotropic temperature factors for each atom. The overall structure of the serotype 2 head is very similar to its serotype 5 homologue, apart from differences in some of the flexible loops. All but subgroup B adenoviruses are believed to use the recently identified protein CAR (Coxsackievirus and adenovirus receptor) as receptor. By comparison of the two structures and sequence alignment of CAR binding and non-CAR binding serotype fiber heads, we discuss possible receptor binding sites and propose a receptor binding site in a crevice between two monomers on the side of the trimer. The structural basis of the extraordinary stability of the fiber head trimer is also discussed.
 ==About this Structure==
-QHV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_adenovirus_2 Human adenovirus 2] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QHV OCA].
+QHV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_adenovirus_2 Human adenovirus 2] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QHV OCA].
 ==Reference==
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 [[Category: Cusack, S.]]
 [[Category: Louis, N.]]
-[[Category: Raaij, M.J.Van.]]
+[[Category: Raaij, M J.Van.]]
 [[Category: SO4]]
 [[Category: extra-ordinary stability]]
 [[Category: receptor binding]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 04:07:08 2007''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:39:44 2008''