Ann Taylor p53 sandbox: Difference between revisions
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1. The amino terminus (1-44) contains the transactivation domain which is responsible for activating downstream target genes. | 1. The amino terminus (1-44) contains the transactivation domain which is responsible for activating downstream target genes. | ||
2. A proline-rich domain (58-101) mediates the p53 response to DNA damage through apoptosis. | 2. A proline-rich domain (58-101) mediates the p53 response to DNA damage through apoptosis. | ||
3. A core DNA-binding domain (102-292), which is the target of 90% of p53 mutations found in human cancers. A single mutation within this domain is sufficient to cause a major conformational change. | 3. A core DNA-binding domain (102-292), which is the target of 90% of p53 mutations found in human cancers. A single mutation within this domain is sufficient to cause a major conformational change. | ||
4. An oligomerization domain (325-256), which contains a β-strand which interacts with another p53 monomer to form a dimer, followed by an α-helix which mediates the dimerization of two p53 dimers to form a tetramer. | 4. An oligomerization domain (325-256), which contains a β-strand which interacts with another p53 monomer to form a dimer, followed by an α-helix which mediates the dimerization of two p53 dimers to form a tetramer. | ||
5. Two nuclear export signals (NES), found within the oligomerization domain, is rich in leucine residues and is highly conserved. It is proposed that oligomerization masks the NES, resulting in p53 remaining in the nucleus. | 5. Two nuclear export signals (NES), found within the oligomerization domain, is rich in leucine residues and is highly conserved. It is proposed that oligomerization masks the NES, resulting in p53 remaining in the nucleus. | ||
6. Three putative nuclear localization signals (NLS) in the C-terminus have been identified via mutagenesis and sequence similarity. | 6. Three putative nuclear localization signals (NLS) in the C-terminus have been identified via mutagenesis and sequence similarity. | ||