1qop: Difference between revisions
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==About this Structure== | ==About this Structure== | ||
1QOP is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with NA, IPL and PLP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ | 1QOP is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with NA, IPL and PLP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20]]. Structure known Active Sites: IPL, NA and PLP. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QOP OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Salmonella typhimurium]] | [[Category: Salmonella typhimurium]] | ||
[[Category: Tryptophan synthase]] | |||
[[Category: Schlichting, I.]] | [[Category: Schlichting, I.]] | ||
[[Category: Weyand, M.]] | [[Category: Weyand, M.]] | ||
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[[Category: tryptophan biosynthesis]] | [[Category: tryptophan biosynthesis]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 10:36:50 2007'' | ||
Revision as of 11:32, 30 October 2007
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CRYSTAL STRUCTURE OF WILD-TYPE TRYPTOPHAN SYNTHASE COMPLEXED WITH INDOLE PROPANOL PHOSPHATE
OverviewOverview
We used freeze trapping to stabilize the Michaelis complex of wild-type, tryptophan synthase and the alpha-subunit substrate indole-3-glycerol, phosphate (IGP) and determined its structure to 1. 8 A resolution. In, addition, we determined the 1.4 A resolution structure of the complex with, indole-3-propanole phosphate (IPP), a noncleavable IGP analogue. The, interaction of the 3'-hydroxyl of IGP with the catalytic alphaGlu49 leads, to a twisting of the propane chain and to a repositioning of the indole, ring compared to IPP. Concomitantly, the catalytic alphaAsp60 rotates, resulting in a translocation of the COMM domain [betaGly102-betaGly189, for definition see Schneider et al. (1998) Biochemistry 37, 5394-5406] in, a direction opposite to the one in the IPP complex. This results in ... [(full description)]
About this StructureAbout this Structure
1QOP is a [Protein complex] structure of sequences from [Salmonella typhimurium] with NA, IPL and PLP as [ligands]. Active as [Tryptophan synthase], with EC number [4.2.1.20]. Structure known Active Sites: IPL, NA and PLP. Full crystallographic information is available from [OCA].
ReferenceReference
Crystal structure of wild-type tryptophan synthase complexed with the natural substrate indole-3-glycerol phosphate., Weyand M, Schlichting I, Biochemistry. 1999 Dec 14;38(50):16469-80. PMID:10600108
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