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== | ==Solution NMR Structure and X-Ray Absorption Analysis of the C-Terminal Zinc-Binding Domain of the SecA ATPase== | ||
The solution NMR structure of a 22-residue Zn(2+)-binding domain (ZBD) | <StructureSection load='1sx0' size='340' side='right'caption='[[1sx0]]' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1sx0]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SX0 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sx0 OCA], [https://pdbe.org/1sx0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sx0 RCSB], [https://www.ebi.ac.uk/pdbsum/1sx0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sx0 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The solution NMR structure of a 22-residue Zn(2+)-binding domain (ZBD) from Esherichia coli preprotein translocase subunit SecA is presented. In conjunction with X-ray absorption analysis, the NMR structure shows that three cysteines and a histidine in the sequence CXCXSGX(8)CH assume a tetrahedral arrangement around the Zn(2+) atom, with an average Zn(2+)-S bond distance of 2.30 A and a Zn(2+)-N bond distance of 2.03 A. The NMR structure shows that ND1 of His20 binds to the Zn(2+) atom. The ND1-Zn(2+) bond is somewhat strained: it makes an angle of approximately 17 degrees with the plane of the ring, and it also shows a significant "in-plane" distortion of 13 degrees. A comprehensive sequence alignment of the SecA-ZBD from many different organisms shows that, along with the four Zn(2+) ligands, there is a serine residue (Ser12) that is completely conserved. The NMR structure indicates that the side chain of this serine residue forms a strong hydrogen bond with the thiolate of the third cysteine residue (Cys19); therefore, the conserved serine appears to have a critical role in the structure. SecB, an export-specific chaperone, is the only known binding partner for the SecA-ZBD. A phylogenetic analysis using 86 microbial genomes shows that 59 of the organisms carry SecA with a ZBD, but only 31 of these organisms also possess a gene for SecB, indicating that there may be uncharacterized binding partners for the SecA-ZBD. | |||
Solution NMR structure and X-ray absorption analysis of the C-terminal zinc-binding domain of the SecA ATPase.,Dempsey BR, Wrona M, Moulin JM, Gloor GB, Jalilehvand F, Lajoie G, Shaw GS, Shilton BH Biochemistry. 2004 Jul 27;43(29):9361-71. PMID:15260479<ref>PMID:15260479</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1sx0" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[SecA|SecA]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Dempsey BR]] | |||
[[Category: Gloor GB]] | |||
[[Category: Jalilehvand F]] | |||
[[Category: Lajoie G]] | |||
[[Category: Moulin JM]] | |||
[[Category: Shaw GS]] | |||
[[Category: Shilton BH]] | |||
[[Category: Wrona M]] | |||
