1g3q: Difference between revisions

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-[[Image:1g3q.gif|left|200px]]<br /><applet load="1g3q" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1g3q, resolution 2.00&Aring;" />
-'''CRYSTAL STRUCTURE ANALYSIS OF PYROCOCCUS FURIOSUS CELL DIVISION ATPASE MIND'''<br />
-==Overview==
+==CRYSTAL STRUCTURE ANALYSIS OF PYROCOCCUS FURIOSUS CELL DIVISION ATPASE MIND==
-Proper placement of the bacterial cell division site requires the, site-specific inactivation of other potential division sites. In, Escherichia coli, selection of the correct mid-cell site is mediated by, the MinC, MinD and MinE proteins. To clarify the functional role of the, bacterial cell division inhibitor MinD, which is a membrane-associated, ATPase that works as an activator of MinC, we determined the crystal, structure of a Pyrococcus furiosus MinD homologue complexed with a, substrate analogue, AMPPCP, and with the product ADP at resolutions of 2.7, and 2.0 A, respectively. The structure reveals general similarities to the, nitrogenase iron protein, the H-Ras p21 and the RecA-like ATPase domain., Alanine scanning mutational analyses of E.coli MinD were also performed in, vivo. The results suggest that the residues around the ATP-binding site, are required for the direct interaction with MinC, and that ATP binding, and hydrolysis play a role as a molecular switch to control the mechanisms, of MinCDE-dependent bacterial cell division.
+<StructureSection load='1g3q' size='340' side='right'caption='[[1g3q]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1g3q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G3Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G3Q FirstGlance]. <br>
-G3Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G3Q OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g3q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g3q OCA], [https://pdbe.org/1g3q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g3q RCSB], [https://www.ebi.ac.uk/pdbsum/1g3q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g3q ProSAT]</span></td></tr>
-Structural and functional studies of MinD ATPase: implications for the molecular recognition of the bacterial cell division apparatus., Hayashi I, Oyama T, Morikawa K, EMBO J. 2001 Apr 17;20(8):1819-28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11296216 11296216]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8U3I1_PYRFU Q8U3I1_PYRFU]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g3/1g3q_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g3q ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Pyrococcus furiosus]]
-[[Category: Single protein]]
+[[Category: Hayashi I]]
-[[Category: Hayashi, I.]]
+[[Category: Morikawa K]]
-[[Category: Morikawa, K.]]
+[[Category: Oyama T]]
-[[Category: Oyama, T.]]
-[[Category: ADP]]
-[[Category: MG]]
-[[Category: alpha-beta-alpha layered]]
-[[Category: protein-adp complex]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:47:30 2007''