2zzf: Difference between revisions

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-{{Seed}}
-[[Image:2zzf.jpg|left|200px]]
-<!--
+==Crystal structure of alanyl-tRNA synthetase without oligomerization domain==
-The line below this paragraph, containing "STRUCTURE_2zzf", creates the "Structure Box" on the page.
+<StructureSection load='2zzf' size='340' side='right'caption='[[2zzf]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2zzf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZZF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZZF FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-{{STRUCTURE_2zzf|  PDB=2zzf  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zzf OCA], [https://pdbe.org/2zzf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zzf RCSB], [https://www.ebi.ac.uk/pdbsum/2zzf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zzf ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SYA_PYRHO SYA_PYRHO] Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Edits incorrectly charged Ser-tRNA(Ala). Incorrectly charged amino acids occur because the of inherent physicochemical limitations on discrimination between closely related amino acids (Gly and Ser) in the charging step.<ref>PMID:16087889</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zz/2zzf_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zzf ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA(Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA(Ala) at 2 different catalytic sites. Here, we describe the monomer structures of C-terminal truncated archaeal AlaRS, with both activation and editing domains in the apo form, in complex with an Ala-AMP analog, and in a high-resolution lysine-methylated form. The structures show docking of the editing domain to the activation domain opposite from the predicted tRNA-binding surface. Thus, the editing site is positioned &gt;35 A from the activation site, prompting us to model 2 different tRNA complexes: one binding tRNA at the activation site, and the other binding tRNA at the editing site. Interestingly, a gel-shift assay also implies the presence of 2 types of tRNA complex with different mobility. These results suggest that tRNA translocation via a canonical CCA flipping is unlikely to occur in AlaRS. The structure also demonstrated the binding of zinc in the editing site, in which the specific coordination of zinc would be facilitated by a conserved GGQ motif, implying that the editing mechanism may not be the same as in ThrRS. As Asn-194 in eubacterial AlaRS important for Ser misactivation is replaced by Thr-213 in archaeal AlaRS, a different Ser accommodation mechanism is proposed.
-===Crystal structure of alanyl-tRNA synthetase without oligomerization domain===
+The structure of alanyl-tRNA synthetase with editing domain.,Sokabe M, Ose T, Nakamura A, Tokunaga K, Nureki O, Yao M, Tanaka I Proc Natl Acad Sci U S A. 2009 Jul 7;106(27):11028-33. Epub 2009 Jun 19. PMID:19549823<ref>PMID:19549823</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2zzf" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_19549823}}, adds the Publication Abstract to the page
+*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 19549823 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_19549823}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-ZZF is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZZF OCA].
-==Reference==
-<ref group="xtra">PMID:19549823</ref><references group="xtra"/>
-[[Category: Alanine--tRNA ligase]]
 [[Category: Pyrococcus horikoshii]]
-[[Category: Nakamura, A.]]
+[[Category: Nakamura A]]
-[[Category: Nureki, O.]]
+[[Category: Nureki O]]
-[[Category: Ose, T.]]
+[[Category: Ose T]]
-[[Category: Sokabe, M.]]
+[[Category: Sokabe M]]
-[[Category: Tanaka, I.]]
+[[Category: Tanaka I]]
-[[Category: Tokunaga, K.]]
+[[Category: Tokunaga K]]
-[[Category: Yao, M.]]
+[[Category: Yao M]]
-[[Category: Hydrolase]]
-[[Category: Ligase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 22 20:33:21 2009''