1jnr: Difference between revisions

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OCA

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-[[Image:1jnr.jpg|left|200px]]<br /><applet load="1jnr" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1jnr, resolution 1.60&Aring;" />
-'''Structure of adenylylsulfate reductase from the hyperthermophilic Archaeoglobus fulgidus at 1.6 resolution'''<br />
-==Overview==
+==Structure of adenylylsulfate reductase from the hyperthermophilic Archaeoglobus fulgidus at 1.6 resolution==
-The iron-sulfur flavoenzyme adenylylsulfate (adenosine 5'-phosphosulfate, APS) reductase catalyzes reversibly the reduction of APS to sulfite and, AMP. The structures of APS reductase from the hyperthermophilic, Archaeoglobus fulgidus in the two-electron reduced state and with sulfite, bound to FAD are reported at 1.6- and 2.5- resolution, respectively. The, FAD-sulfite adduct was detected after soaking the crystals with APS. This, finding and the architecture of the active site strongly suggest that, catalysis involves a nucleophilic attack of the N5 atom of reduced FAD on, the sulfur atom of APS. In view of the high degree of similarity between, APS reductase and fumarate reductase especially with regard to the, FAD-binding alpha-subunit, it is proposed that both subunits originate, from a common ancestor resembling archaeal APS reductase. The two, electrons required for APS reduction are transferred via two [4Fe-4S], clusters from the surface of the protein to FAD. The exceptionally large, difference in reduction potential of these clusters (-60 and -500 mV) can, be explained by interactions of the clusters with the protein matrix.
+<StructureSection load='1jnr' size='340' side='right'caption='[[1jnr]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1jnr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JNR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JNR FirstGlance]. <br>
-JNR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus] with FAD, SF4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenylyl-sulfate_reductase Adenylyl-sulfate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.99.2 1.8.99.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JNR OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jnr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jnr OCA], [https://pdbe.org/1jnr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jnr RCSB], [https://www.ebi.ac.uk/pdbsum/1jnr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jnr ProSAT]</span></td></tr>
-Structure of adenylylsulfate reductase from the hyperthermophilic Archaeoglobus fulgidus at 1.6-A resolution., Fritz G, Roth A, Schiffer A, Buchert T, Bourenkov G, Bartunik HD, Huber H, Stetter KO, Kroneck PM, Ermler U, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1836-41. Epub 2002 Feb 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11842205 11842205]
+</table>
-[[Category: Adenylyl-sulfate reductase]]
+== Function ==
-[[Category: Archaeoglobus fulgidus]]
+[https://www.uniprot.org/uniprot/O28603_ARCFU O28603_ARCFU]
-[[Category: Protein complex]]
+== Evolutionary Conservation ==
-[[Category: Bartunik, H.D.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Bourenkov, G.]]
+Check<jmol>
-[[Category: Buechert, T.]]
+  <jmolCheckbox>
-[[Category: Ermler, U.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jn/1jnr_consurf.spt"</scriptWhenChecked>
-[[Category: Fritz, G.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: Huber, H.]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: Kroneck, P.M.H.]]
+  </jmolCheckbox>
-[[Category: Roth, A.]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jnr ConSurf].
-[[Category: Schiffer, A.]]
+<div style="clear:both"></div>
-[[Category: Stetter, K.O.]]
+__TOC__
-[[Category: FAD]]
+</StructureSection>
-[[Category: GOL]]
+[[Category: Archaeoglobus fulgidus DSM 4304]]
-[[Category: SF4]]
+[[Category: Large Structures]]
-[[Category: sulfur metabolism/ adenylylsulfate reductase/ iron-sulfur flavoprotein/ crystal structure/catalysis]]
+[[Category: Bartunik HD]]
+[[Category: Bourenkov G]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:55:16 2007''
+[[Category: Buechert T]]
+[[Category: Ermler U]]
+[[Category: Fritz G]]
+[[Category: Huber H]]
+[[Category: Kroneck PMH]]
+[[Category: Roth A]]
+[[Category: Schiffer A]]
+[[Category: Stetter KO]]