User:Tilman Schirmer/Sandbox 204: Difference between revisions
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== Overview == | == Overview == | ||
<applet load='2bre' scene='User:Tilman_Schirmer/Sandbox_204/Protomer/3' size='300' frame='true' align='right' caption='WspR | <applet load='2bre' scene='User:Tilman_Schirmer/Sandbox_204/Protomer/3' size='300' frame='true' align='right' caption='WspR [[3bre]]' /> | ||
<scene name='User:Tilman_Schirmer/Sandbox_204/Protomer/3'>WspR </scene> from ''Pseudomonas aeruginosa'' is a response regulator with an catalytic, diguanylate cyclase, output domain. It is composed of a canonical CheY-like response regulator receiver domain (<scene name='User:Tilman_Schirmer/Sandbox_204/Rec/2'>Rec</scene>) and a C-terminal <scene name='User:Tilman_Schirmer/Sandbox_204/Ggdef/2'>catalytic GGDEF domain</scene> domain that confers the catalytic activity with all canonical <scene name='User:Tilman_Schirmer/Sandbox_204/Substrate_binding_site/2'>active site residues</scene> present. | <scene name='User:Tilman_Schirmer/Sandbox_204/Protomer/3'>WspR </scene> from ''Pseudomonas aeruginosa'' is a response regulator with an catalytic, diguanylate cyclase, output domain. It is composed of a canonical CheY-like response regulator receiver domain (<scene name='User:Tilman_Schirmer/Sandbox_204/Rec/2'>Rec</scene>) and a C-terminal <scene name='User:Tilman_Schirmer/Sandbox_204/Ggdef/2'>catalytic GGDEF domain</scene> domain that confers the catalytic activity with all canonical <scene name='User:Tilman_Schirmer/Sandbox_204/Substrate_binding_site/2'>active site residues</scene> present. | ||
<br>Although not modified (i.e. phosphorylated) at the active Asp (Asp70), the Rec domains mediate formation of <scene name='User:Tilman_Schirmer/Sandbox_204/Dimer/1'>dimeric </scene> WspR. Two dimers, in turn, are associated by head-to-head contact to a <scene name='User:Tilman_Schirmer/Sandbox_204/Tetramer/1'>tetramer</scene> of approximate 222 (D2) symmetry. | <br>Although not modified (i.e. phosphorylated) at the active Asp (<scene name='User:Tilman_Schirmer/Sandbox_204/Rec/4'>Asp70</scene>), the Rec domains mediate formation of <scene name='User:Tilman_Schirmer/Sandbox_204/Dimer/1'>dimeric </scene> WspR. Two dimers, in turn, are associated by head-to-head contact to a <scene name='User:Tilman_Schirmer/Sandbox_204/Tetramer/1'>tetramer</scene> of approximate 222 (D2) symmetry. | ||
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== Allosteric product binding site == | == Allosteric product binding site == | ||
<applet load='2bre' scene='User:Tilman_Schirmer/Sandbox_204/Ip/3' size='300' frame='true' align='right' caption='WspR | <applet load='2bre' scene='User:Tilman_Schirmer/Sandbox_204/Ip/3' size='300' frame='true' align='right' caption='WspR [[3bre]]' /> | ||
There are two allosteric sites (<scene name='User:Tilman_Schirmer/Sandbox_204/Ip/3'>primary inhibition site, Ip,</scene> and <scene name='User:Tilman_Schirmer/Sandbox_204/Is/3'>secondary inhibition site, Is</scene>) that | There are two allosteric sites (<scene name='User:Tilman_Schirmer/Sandbox_204/Ip/3'>primary inhibition site, Ip,</scene> and <scene name='User:Tilman_Schirmer/Sandbox_204/Is/3'>secondary inhibition site, Is</scene>) that become cross-linked by (c-di-GMP)<sub>2</sub> dimers in the <scene name='User:Tilman_Schirmer/Sandbox_204/Tetramer/5'>tetrameric</scene> molecule. For a close-up click <scene name='User:Tilman_Schirmer/Sandbox_204/Tetramer/4'>here</scene> (<scene name='User:Tilman_Schirmer/Sandbox_204/Tetramer/6'>Ip-site</scene>, <scene name='User:Tilman_Schirmer/Sandbox_204/Tetramer/8'>Is-site</scene>, <scene name='User:Tilman_Schirmer/Sandbox_204/Tetramer/9'>(c-di-GMP dimer)2</scene>). Note that there are four (c-di-GMP)<sub>2</sub> dimers per WspR tetramer. | ||
<br><br><br><br> | <br><br><br><br> | ||
==References== | |||
WspR structure [[3bre]]: | |||
<ref group="xtra">PMID:18366254</ref> | |||
<references group="xtra"/> | |||