1mws: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1mws.jpg|left|200px]]<br /><applet load="1mws" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1mws, resolution 2.00&Aring;" />
-'''Structure of nitrocefin acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2.00 A resolution.'''<br />
-==Overview==
+==Structure of nitrocefin acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2.00 A resolution.==
-The multiple antibiotic resistance of methicillin-resistant strains of, Staphylococcus aureus (MRSA) has become a major clinical problem, worldwide. The key determinant of the broad-spectrum beta-lactam, resistance in MRSA strains is the penicillin-binding protein 2a (PBP2a)., Because of its low affinity for beta-lactams, PBP2a provides, transpeptidase activity to allow cell wall synthesis at beta-lactam, concentrations that inhibit the beta-lactam-sensitive PBPs normally, produced by S. aureus. The crystal structure of a soluble derivative of, PBP2a has been determined to 1.8 A resolution and provides the highest, resolution structure for a high molecular mass PBP. Additionally, structures of the acyl-PBP complexes of PBP2a with nitrocefin, penicillin, G and methicillin allow, for the first time, a comparison of an apo and, acylated resistant PBP. An analysis of the PBP2a active site in these, forms reveals the structural basis of its resistance and identifies, features in newly developed beta-lactams that are likely important for, high affinity binding.
+<StructureSection load='1mws' size='340' side='right'caption='[[1mws]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1mws]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWS FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NC1:NITROCEFIN+ACYL-SERINE'>NC1</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mws OCA], [https://pdbe.org/1mws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mws RCSB], [https://www.ebi.ac.uk/pdbsum/1mws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mws ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q93IC2_STAAU Q93IC2_STAAU]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mw/1mws_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mws ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-MWS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with CD and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA].
+*[[Penicillin-binding protein 3D structures|Penicillin-binding protein 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Structural basis for the beta lactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus., Lim D, Strynadka NC, Nat Struct Biol. 2002 Nov;9(11):870-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12389036 12389036]
+[[Category: Large Structures]]
-[[Category: Single protein]]
 [[Category: Staphylococcus aureus]]
-[[Category: Lim, D.C.]]
+[[Category: Lim DC]]
-[[Category: Strynadka, N.C.J.]]
+[[Category: Strynadka NCJ]]
-[[Category: CD]]
-[[Category: CL]]
-[[Category: beta-lactam]]
-[[Category: d]]
-[[Category: d-carboxypeptidase]]
-[[Category: d-transpeptidase]]
-[[Category: nitrocefin]]
-[[Category: penicillin binding protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:08:23 2007''