2iwm: Difference between revisions

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New page: left|200px<br /><applet load="2iwm" size="450" color="white" frame="true" align="right" spinBox="true" caption="2iwm, resolution 2.50Å" /> '''PRECURSOR MUTANT CYS...
 
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[[Image:2iwm.jpg|left|200px]]<br /><applet load="2iwm" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2iwm, resolution 2.50&Aring;" />
'''PRECURSOR MUTANT CYS1SER OF PENICILLIN V ACYLASE FROM BACILLUS SPHAERICUS'''<br />


==Overview==
==precursor mutant Cys1Ser of Penicillin V Acylase from Bacillus sphaericus==
The crystallization of three catalytically inactive mutants of penicillin, V acylase (PVA) from Bacillus sphaericus in precursor and processed forms, is reported. The mutant proteins crystallize in different primitive, monoclinic space groups that are distinct from the crystal forms for the, native enzyme. Directed mutants and clone constructs were designed to, study the post-translational autoproteolytic processing of PVA. The, catalytically inactive mutants will provide three-dimensional structures, of precursor PVA forms, plus open a route to the study of enzyme-substrate, complexes for this industrially important enzyme.
<StructureSection load='2iwm' size='340' side='right'caption='[[2iwm]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2iwm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IWM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iwm OCA], [https://pdbe.org/2iwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iwm RCSB], [https://www.ebi.ac.uk/pdbsum/2iwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iwm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PAC_LYSSH PAC_LYSSH] The enzyme catalyzes the conversion of penicillin to 6-aminopenicillanate The precursor, furthermore, acts as a self-processing peptidase that cleaves off the propeptide. All peptidase activity is lost on conversion to the mature peptidase.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iw/2iwm_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iwm ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
2IWM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lysinibacillus_sphaericus Lysinibacillus sphaericus]. Active as [http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IWM OCA].
*[[Penicillin acylase|Penicillin acylase]]
 
__TOC__
==Reference==
</StructureSection>
Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants., Chandra PM, Brannigan JA, Prabhune A, Pundle A, Turkenburg JP, Dodson GG, Suresh CG, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):124-7. Epub 2004 Dec 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16508111 16508111]
[[Category: Large Structures]]
[[Category: Lysinibacillus sphaericus]]
[[Category: Lysinibacillus sphaericus]]
[[Category: Penicillin amidase]]
[[Category: Chandra PM]]
[[Category: Single protein]]
[[Category: Dodson G]]
[[Category: Chandra, P.M.]]
[[Category: Suresh CG]]
[[Category: Dodson, G.]]
[[Category: Suresh, C.G.]]
[[Category: antibiotic resistance]]
[[Category: autoproteolysis]]
[[Category: hydrolase]]
[[Category: penicillin]]
[[Category: precursor]]
[[Category: zymogen]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 12:27:11 2007''

Latest revision as of 17:27, 13 December 2023

precursor mutant Cys1Ser of Penicillin V Acylase from Bacillus sphaericusprecursor mutant Cys1Ser of Penicillin V Acylase from Bacillus sphaericus

Structural highlights

2iwm is a 4 chain structure with sequence from Lysinibacillus sphaericus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PAC_LYSSH The enzyme catalyzes the conversion of penicillin to 6-aminopenicillanate The precursor, furthermore, acts as a self-processing peptidase that cleaves off the propeptide. All peptidase activity is lost on conversion to the mature peptidase.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2iwm, resolution 2.50Å

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