1osd: Difference between revisions

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-{{Seed}}
-[[Image:1osd.png|left|200px]]
-<!--
+==crystal structure of Oxidized MerP from Ralstonia metallidurans CH34==
-The line below this paragraph, containing "STRUCTURE_1osd", creates the "Structure Box" on the page.
+<StructureSection load='1osd' size='340' side='right'caption='[[1osd]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1osd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_metallidurans Cupriavidus metallidurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OSD FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1osd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1osd OCA], [https://pdbe.org/1osd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1osd RCSB], [https://www.ebi.ac.uk/pdbsum/1osd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1osd ProSAT]</span></td></tr>
-{{STRUCTURE_1osd|  PDB=1osd  |  SCENE=  }}
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/os/1osd_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1osd ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+In Ralstonia metallidurans CH34, the gene merP encodes for a periplasmic mercury-binding protein which is capable of binding one mercury atom. The metal-binding site of MerP consists of the highly conserved sequence GMTCXXC found in the family that includes metallochaperones and metal-transporting ATPases. We purified MerP from R.metallidurans CH34 and solved its crystal structure under the oxidized form at 2.0A resolution. Superposition with structures of other metal-binding proteins shows that the global structure of R.metallidurans CH34 oxidized MerP follows the general topology of the whole family. The largest differences are observed with the NMR structure of oxidized Shigella flexneri MerP. Detailed analysis of the metal-binding site suggests a direct role for Y66 in stabilizing the thiolate group of C17 during the mercury-binding reaction. The metal-binding site of oxidized MerP is also similar to the metal-binding sites of oxidized copper chaperone for superoxide dismutase and Atx1, two copper-binding proteins from Saccharomyces cerevisiae. Finally, the packing of the MerP crystals suggests that F38, a well-conserved residue in the MerP family may be important in mercury binding and transfer. We propose a possible mechanism of mercury transfer between two CXXC motifs based on a transient bi-coordinated mercury intermediate.
-===crystal structure of Oxidized MerP from Ralstonia metallidurans CH34===
+Crystal structure of the oxidized form of the periplasmic mercury-binding protein MerP from Ralstonia metallidurans CH34.,Serre L, Rossy E, Pebay-Peyroula E, Cohen-Addad C, Coves J J Mol Biol. 2004 May 21;339(1):161-71. PMID:15123428<ref>PMID:15123428</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_15123428}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1osd" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 15123428 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_15123428}}
+__TOC__
+</StructureSection>
-==About this Structure==
-OSD is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Cupriavidus_metallidurans Cupriavidus metallidurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSD OCA].
-==Reference==
-<ref group="xtra">PMID:15123428</ref><references group="xtra"/>
 [[Category: Cupriavidus metallidurans]]
-[[Category: Cohen-Addad, C.]]
+[[Category: Large Structures]]
-[[Category: Coves, J.]]
+[[Category: Cohen-Addad C]]
-[[Category: Pebay-Peyroula, E.]]
+[[Category: Coves J]]
-[[Category: Rossy, E.]]
+[[Category: Pebay-Peyroula E]]
-[[Category: Serre, L.]]
+[[Category: Rossy E]]
-[[Category: Mercury resistance]]
+[[Category: Serre L]]
-[[Category: Metal binding protein]]
-[[Category: Perisplasm]]
-[[Category: Structural genomic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:44:15 2009''