2hj6: Difference between revisions

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New page: left|200px<br /><applet load="2hj6" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hj6, resolution 3.00Å" /> '''Reaction centre from...
 
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[[Image:2hj6.gif|left|200px]]<br /><applet load="2hj6" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2hj6, resolution 3.00&Aring;" />
'''Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylserine'''<br />


==Overview==
==Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylserine==
This study describes the use of brominated phospholipids to distinguish, between lipid and detergent binding sites on the surface of a typical, alpha-helical membrane protein. Reaction centers isolated from Rhodobacter, sphaeroides were cocrystallized with added brominated phospholipids. X-ray, structural analysis of these crystals has revealed the presence of two, lipid binding sites from the characteristic strong X-ray scattering from, the bromine atoms. These results demonstrate the usefulness of this, approach to mapping lipid binding sites at the surface of membrane, proteins.
<StructureSection load='2hj6' size='340' side='right'caption='[[2hj6]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2hj6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HJ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HJ6 FirstGlance]. <br>
2HJ6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with FE, K, PO4, BCL, BPH, U10, HTO, CDL, PS2, LDA and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2HJ6 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PS2:O-[{[(2R)-2-{[(9S,10S)-9,10-DIBROMOOCTADECANOYL]OXY}-3-(PALMITOYLOXY)PROPYL]OXY}(HYDROXY)PHOSPHORYL]-L-SERINE'>PS2</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hj6 OCA], [https://pdbe.org/2hj6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hj6 RCSB], [https://www.ebi.ac.uk/pdbsum/2hj6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hj6 ProSAT]</span></td></tr>
Brominated Lipids Identify Lipid Binding Sites on the Surface of the Reaction Center from Rhodobacter sphaeroides., Roszak AW, Gardiner AT, Isaacs NW, Cogdell RJ, Biochemistry. 2007 Mar 20;46(11):2909-16. Epub 2007 Feb 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17315985 17315985]
</table>
[[Category: Protein complex]]
== Function ==
[[Category: Rhodobacter sphaeroides]]
[https://www.uniprot.org/uniprot/RCEL_CERSP RCEL_CERSP] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
[[Category: Cogdell, R.J.]]
== Evolutionary Conservation ==
[[Category: Gardiner, A.T.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Isaacs, N.W.]]
Check<jmol>
[[Category: Roszak, A.W.]]
  <jmolCheckbox>
[[Category: BCL]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/2hj6_consurf.spt"</scriptWhenChecked>
[[Category: BPH]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: CDL]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: FE]]
  </jmolCheckbox>
[[Category: GOL]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hj6 ConSurf].
[[Category: HTO]]
<div style="clear:both"></div>
[[Category: K]]
__TOC__
[[Category: LDA]]
</StructureSection>
[[Category: PO4]]
[[Category: Cereibacter sphaeroides]]
[[Category: PS2]]
[[Category: Large Structures]]
[[Category: U10]]
[[Category: Cogdell RJ]]
[[Category: brominated lipids]]
[[Category: Gardiner AT]]
[[Category: lipid binding sites]]
[[Category: Isaacs NW]]
[[Category: membrane protein]]
[[Category: Roszak AW]]
[[Category: photosynthesis]]
[[Category: photosynthetic reaction center]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:48:17 2007''

Latest revision as of 12:33, 14 February 2024

Reaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylserineReaction centre from Rhodobacter sphaeroides strain R-26.1 complexed with dibrominated phosphatidylserine

Structural highlights

2hj6 is a 3 chain structure with sequence from Cereibacter sphaeroides. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3Å
Ligands:, , , , , , , , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RCEL_CERSP The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

2hj6, resolution 3.00Å

Drag the structure with the mouse to rotate

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OCA
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