1ji2: Difference between revisions

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[[Image:1ji2.png|left|200px]]


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==Improved X-ray Structure of Thermoactinomyces vulgaris R-47 alpha-Amylase 2==
The line below this paragraph, containing "STRUCTURE_1ji2", creates the "Structure Box" on the page.
<StructureSection load='1ji2' size='340' side='right'caption='[[1ji2]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1ji2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoactinomyces_vulgaris Thermoactinomyces vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JI2 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
{{STRUCTURE_1ji2| PDB=1ji2 |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ji2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ji2 OCA], [https://pdbe.org/1ji2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ji2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ji2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ji2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NEPU2_THEVU NEPU2_THEVU] Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ji/1ji2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ji2 ConSurf].
<div style="clear:both"></div>


===Improved X-ray Structure of Thermoactinomyces vulgaris R-47 alpha-Amylase 2===
==See Also==
 
*[[Amylase 3D structures|Amylase 3D structures]]
 
__TOC__
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[[Category: Large Structures]]
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{{ABSTRACT_PUBMED_12051850}}
 
==About this Structure==
1JI2 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermoactinomyces_vulgaris Thermoactinomyces vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI2 OCA].
 
==Reference==
<ref group="xtra">PMID:12051850</ref><references group="xtra"/>
[[Category: Neopullulanase]]
[[Category: Thermoactinomyces vulgaris]]
[[Category: Thermoactinomyces vulgaris]]
[[Category: Abe, A.]]
[[Category: Abe A]]
[[Category: Kaji, A.]]
[[Category: Kaji A]]
[[Category: Kamitori, S.]]
[[Category: Kamitori S]]
[[Category: Ohtaki, A.]]
[[Category: Ohtaki A]]
[[Category: Sakano, Y.]]
[[Category: Sakano Y]]
[[Category: Tonozuka, T.]]
[[Category: Tonozuka T]]
[[Category: Beta/alpha barrel]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 21:45:15 2009''

Latest revision as of 16:24, 13 March 2024

Improved X-ray Structure of Thermoactinomyces vulgaris R-47 alpha-Amylase 2Improved X-ray Structure of Thermoactinomyces vulgaris R-47 alpha-Amylase 2

Structural highlights

1ji2 is a 2 chain structure with sequence from Thermoactinomyces vulgaris. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NEPU2_THEVU Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1ji2, resolution 2.30Å

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