1m8m: Difference between revisions

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-{{Seed}}
-[[Image:1m8m.png|left|200px]]
-<!--
+==SOLID-STATE MAS NMR STRUCTURE OF THE A-SPECTRIN SH3 DOMAIN==
-The line below this paragraph, containing "STRUCTURE_1m8m", creates the "Structure Box" on the page.
+<StructureSection load='1m8m' size='340' side='right'caption='[[1m8m]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1m8m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M8M FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8m OCA], [https://pdbe.org/1m8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m8m RCSB], [https://www.ebi.ac.uk/pdbsum/1m8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m8m ProSAT]</span></td></tr>
-{{STRUCTURE_1m8m|  PDB=1m8m  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SPTN1_CHICK SPTN1_CHICK] Morphologically, spectrin-like proteins appear to be related to spectrin, showing a flexible rod-like structure. They can bind actin but seem to differ in their calmodulin-binding activity. In nonerythroid tissues, spectrins, in association with some other proteins, may play an important role in membrane organization.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m8/1m8m_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m8m ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The determination of a representative set of protein structures is a chief aim in structural genomics. Solid-state NMR may have a crucial role in structural investigations of those proteins that do not easily form crystals or are not accessible to solution NMR, such as amyloid systems or membrane proteins. Here we present a protein structure determined by solid-state magic-angle-spinning (MAS) NMR. Almost complete (13)C and (15)N resonance assignments for a micro-crystalline preparation of the alpha-spectrin Src-homology 3 (SH3) domain formed the basis for the extraction of a set of distance restraints. These restraints were derived from proton-driven spin diffusion (PDSD) spectra of biosynthetically site-directed, labelled samples obtained from bacteria grown using [1,3-(13)C]glycerol or [2-(13)C]glycerol as carbon sources. This allowed the observation of long-range distance correlations up to approximately 7 A. The calculated global fold of the alpha-spectrin SH3 domain is based on 286 inter-residue (13)C-(13)C and six (15)N-(15)N restraints, all self-consistently obtained by solid-state MAS NMR. This MAS NMR procedure should be widely applicable to small membrane proteins that can be expressed in bacteria.
-===SOLID-STATE MAS NMR STRUCTURE OF THE A-SPECTRIN SH3 DOMAIN===
+Structure of a protein determined by solid-state magic-angle-spinning NMR spectroscopy.,Castellani F, van Rossum B, Diehl A, Schubert M, Rehbein K, Oschkinat H Nature. 2002 Nov 7;420(6911):98-102. PMID:12422222<ref>PMID:12422222</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1m8m" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_12422222}}, adds the Publication Abstract to the page
+*[[Spectrin 3D structures|Spectrin 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 12422222 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_12422222}}
+__TOC__
+</StructureSection>
-==About this Structure==
-M8M is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8M OCA].
-==Reference==
-<ref group="xtra">PMID:12422222</ref><references group="xtra"/>
 [[Category: Gallus gallus]]
-[[Category: Castellani, F.]]
+[[Category: Large Structures]]
-[[Category: Diehl, A.]]
+[[Category: Castellani F]]
-[[Category: Oschkinat, H.]]
+[[Category: Diehl A]]
-[[Category: Rehbein, K.]]
+[[Category: Oschkinat H]]
-[[Category: Rossum, B Van.]]
+[[Category: Rehbein K]]
-[[Category: Schubert, M.]]
+[[Category: Schubert M]]
-[[Category: Solid-state mas nmr structure]]
+[[Category: Van Rossum B]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:28:46 2009''