2f68: Difference between revisions

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OCA

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-[[Image:2f68.gif|left|200px]]<br /><applet load="2f68" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2f68, resolution 1.95&Aring;" />
-'''Crystal structure of collagen adhesin (CNA) from S. aureus'''<br />
-==Overview==
+==Crystal structure of collagen adhesin (CNA) from S. aureus==
-The structural basis for the association of eukaryotic and prokaryotic, protein receptors and their triple-helical collagen ligand remains poorly, understood. Here, we present the crystal structures of a high affinity, subsegment of the Staphylococcus aureus collagen-binding CNA as an, apo-protein and in complex with a synthetic collagen-like triple helical, peptide. The apo-protein structure is composed of two subdomains (N1 and, N2), each adopting a variant IgG-fold, and a long linker that connects N1, and N2. The structure is stabilized by hydrophobic inter-domain, interactions and by the N2 C-terminal extension that complements a, beta-sheet on N1. In the ligand complex, the collagen-like peptide, penetrates through a spherical hole formed by the two subdomains and the, N1-N2 linker. Based on these two structures we propose a dynamic, multistep binding model, called the 'Collagen Hug' that is uniquely, designed to allow multidomain collagen binding proteins to bind their, extended rope-like ligand.
+<StructureSection load='2f68' size='340' side='right'caption='[[2f68]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2f68]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F68 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f68 OCA], [https://pdbe.org/2f68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f68 RCSB], [https://www.ebi.ac.uk/pdbsum/2f68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f68 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CNA_STAAU CNA_STAAU] Mediates attachment of staphylococcal cells to collagen-containing substrata.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f6/2f68_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f68 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-F68 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2F68 OCA].
+*[[Adhesin 3D structures|Adhesin 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-A 'Collagen Hug' model for Staphylococcus aureus CNA binding to collagen., Zong Y, Xu Y, Liang X, Keene DR, Hook A, Gurusiddappa S, Hook M, Narayana SV, EMBO J. 2005 Dec 21;24(24):4224-36. Epub 2005 Dec 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16362049 16362049]
+[[Category: Large Structures]]
-[[Category: Single protein]]
 [[Category: Staphylococcus aureus]]
-[[Category: Narayana, S.V.L.]]
+[[Category: Narayana SVL]]
-[[Category: Zong, Y.]]
+[[Category: Zong Y]]
-[[Category: beta barrel]]
-[[Category: domain swap]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:23:15 2007''