1h9c: Difference between revisions

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New page: left|200px<br /> <applet load="1h9c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h9c" /> '''NMR STRUCTURE OF CYSTEINYL-PHOSPHORYLATED E...
 
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[[Image:1h9c.gif|left|200px]]<br />
<applet load="1h9c" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1h9c" />
'''NMR STRUCTURE OF CYSTEINYL-PHOSPHORYLATED ENZYME IIB OF THE N,N'-DIACETYLCHITOBIOSE SPECIFIC PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM OF ESCHERICHIA COLI.'''<br />


==Overview==
==NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli.==
The determination by NMR of the solution structure of the phosphorylated, enzyme IIB (P-IIB(Chb)) of the N,N'-diacetylchitobiose-specific, phosphoenolpyruvate-dependent phosphotransferase system of Escherichia, coli is presented. Most of the backbone and side-chain resonances were, assigned using a variety of mostly heteronuclear NMR experiments. The, remaining resonances were assigned with the help of the structure, calculations.NOE-derived distance restraints were used in distance, geometry calculations followed by molecular dynamics and simulated, annealing protocols. In addition, combinations of ambiguous restraints, were used to resolve ambiguities in the NOE assignments. By combining sets, of ambiguous and unambiguous restraints into new ambiguous restraints, an, error function was ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11352587 (full description)]]
<StructureSection load='1h9c' size='340' side='right'caption='[[1h9c]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1h9c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._W3110 Escherichia coli str. K-12 substr. W3110]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H9C FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSP:S-PHOSPHOCYSTEINE'>CSP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h9c OCA], [https://pdbe.org/1h9c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h9c RCSB], [https://www.ebi.ac.uk/pdbsum/1h9c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h9c ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PTQB_ECOLI PTQB_ECOLI] The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N,N'-diacetylchitobiose transport.<ref>PMID:10913117</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h9/1h9c_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h9c ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1H9C is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H9C OCA]].
*[[Phosphotransferase 3D structures|Phosphotransferase 3D structures]]
 
== References ==
==Reference==
<references/>
NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose-specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli., Ab E, Schuurman-Wolters GK, Nijlant D, Dijkstra K, Saier MH, Robillard GT, Scheek RM, J Mol Biol. 2001 May 18;308(5):993-1009. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11352587 11352587]
__TOC__
[[Category: Escherichia coli]]
</StructureSection>
[[Category: Single protein]]
[[Category: Escherichia coli str. K-12 substr. W3110]]
[[Category: Ab, E.]]
[[Category: Large Structures]]
[[Category: Dijkstra, K.]]
[[Category: Ab E]]
[[Category: Nijlant, D.]]
[[Category: Dijkstra K]]
[[Category: Robillard, G.T.]]
[[Category: Nijlant D]]
[[Category: Saier, M.H.]]
[[Category: Robillard GT]]
[[Category: Scheek, R.M.]]
[[Category: Saier MH]]
[[Category: Schuurman-Wolters, G.]]
[[Category: Scheek RM]]
[[Category: enzyme iib-chitobiose]]
[[Category: Schuurman-Wolters GK]]
[[Category: iib-cellobiose]]
[[Category: phosphorylation]]
[[Category: phosphotransferase system]]
[[Category: sugar transport]]
[[Category: transferase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 21:52:47 2007''

Latest revision as of 14:29, 27 March 2024

NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli.NMR structure of cysteinyl-phosphorylated enzyme IIB of the N,N'-diacetylchitobiose specific phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli.

Structural highlights

1h9c is a 1 chain structure with sequence from Escherichia coli str. K-12 substr. W3110. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PTQB_ECOLI The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in N,N'-diacetylchitobiose transport.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Keyhani NO, Wang LX, Lee YC, Roseman S. The chitin disaccharide, N,N'-diacetylchitobiose, is catabolized by Escherichia coli and is transported/phosphorylated by the phosphoenolpyruvate:glycose phosphotransferase system. J Biol Chem. 2000 Oct 20;275(42):33084-90. PMID:10913117 doi:http://dx.doi.org/10.1074/jbc.M001043200
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