2nz2: Difference between revisions

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-{{Seed}}
-[[Image:2nz2.png|left|200px]]
-<!--
+==Crystal structure of human argininosuccinate synthase in complex with aspartate and citrulline==
-The line below this paragraph, containing "STRUCTURE_2nz2", creates the "Structure Box" on the page.
+<StructureSection load='2nz2' size='340' side='right'caption='[[2nz2]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2nz2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NZ2 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene>, <scene name='pdbligand=CIR:CITRULLINE'>CIR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-{{STRUCTURE_2nz2|  PDB=2nz2  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nz2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nz2 OCA], [https://pdbe.org/2nz2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nz2 RCSB], [https://www.ebi.ac.uk/pdbsum/2nz2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nz2 ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/ASSY_HUMAN ASSY_HUMAN] Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:[https://omim.org/entry/215700 215700]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN1 usually manifests in the first few days of life. Affected infants appear normal at birth, but as ammonia builds up in the body they present symptoms such as lethargy, poor feeding, vomiting, seizures and loss of consciousness. Less commonly, a milder CTLN1 form can develop later in childhood or adulthood.<ref>PMID:11941481</ref> <ref>PMID:2358466</ref> <ref>PMID:1943692</ref> <ref>PMID:7977368</ref> <ref>PMID:8792870</ref> <ref>PMID:11708871</ref> <ref>PMID:12815590</ref> <ref>PMID:14680976</ref> <ref>PMID:16475226</ref>
+== Function ==
+[https://www.uniprot.org/uniprot/ASSY_HUMAN ASSY_HUMAN]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nz/2nz2_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nz2 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Argininosuccinate synthetase catalyzes the transformation of citrulline and aspartate into argininosuccinate and pyrophosphate using the hydrolysis of ATP to AMP and pyrophosphate. This enzymatic process constitutes the rate-limiting step in both the urea and arginine-citrulline cycles. Previous studies have investigated the crystal structures of argininosuccinate synthetase from bacterial species. In this work, the first crystal structure of human argininosuccinate synthetase in complex with the substrates citrulline and aspartate is presented. The human enzyme is compared with structures of argininosuccinate synthetase from bacteria. In addition, the structure also provides new insights into the function of the numerous clinical mutations identified in patients with type I citrullinaemia (also known as classic citrullinaemia).
-===Crystal structure of human argininosuccinate synthase in complex with aspartate and citrulline===
+Structure of human argininosuccinate synthetase.,Karlberg T, Collins R, van den Berg S, Flores A, Hammarstrom M, Hogbom M, Holmberg Schiavone L, Uppenberg J Acta Crystallogr D Biol Crystallogr. 2008 Mar;64(Pt 3):279-86. Epub 2008, Feb 20. PMID:18323623<ref>PMID:18323623</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-==About this Structure==
+</div>
-NZ2 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NZ2 OCA].
+<div class="pdbe-citations 2nz2" style="background-color:#fffaf0;"></div>
-[[Category: Argininosuccinate synthase]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Arrowsmith, C.]]
+[[Category: Large Structures]]
-[[Category: Berg, S Van Den.]]
+[[Category: Arrowsmith C]]
-[[Category: Berglund, H.]]
+[[Category: Berglund H]]
-[[Category: Busam, R D.]]
+[[Category: Busam RD]]
-[[Category: Collins, R.]]
+[[Category: Collins R]]
-[[Category: Edwards, A.]]
+[[Category: Edwards A]]
-[[Category: Ericsson, U B.]]
+[[Category: Ericsson UB]]
-[[Category: Flodin, S.]]
+[[Category: Flodin S]]
-[[Category: Flores, A.]]
+[[Category: Flores A]]
-[[Category: Graslund, S.]]
+[[Category: Graslund S]]
-[[Category: Hallberg, B M.]]
+[[Category: Hallberg BM]]
-[[Category: Hammarstrom, M.]]
+[[Category: Hammarstrom M]]
-[[Category: Hogbom, M.]]
+[[Category: Hogbom M]]
-[[Category: Holmberg-Schiavone, L.]]
+[[Category: Holmberg-Schiavone L]]
-[[Category: Johansson, I.]]
+[[Category: Johansson I]]
-[[Category: Karlberg, T.]]
+[[Category: Karlberg T]]
-[[Category: Kotenyova, T.]]
+[[Category: Kotenyova T]]
-[[Category: Magnusdottir, A.]]
+[[Category: Magnusdottir A]]
-[[Category: Moche, M.]]
+[[Category: Moche M]]
-[[Category: Nilsson, M E.]]
+[[Category: Nilsson ME]]
-[[Category: Nordlund, P.]]
+[[Category: Nordlund P]]
-[[Category: Nyman, T.]]
+[[Category: Nyman T]]
-[[Category: Ogg, D.]]
+[[Category: Ogg D]]
-[[Category: Persson, C.]]
+[[Category: Persson C]]
-[[Category: SGC, Structural Genomics Consortium.]]
+[[Category: Sagemark J]]
-[[Category: Sagemark, J.]]
+[[Category: Stenmark P]]
-[[Category: Stenmark, P.]]
+[[Category: Sundstrom M]]
-[[Category: Sundstrom, M.]]
+[[Category: Thorsell AG]]
-[[Category: Thorsell, A G.]]
+[[Category: Uppenberg J]]
-[[Category: Uppenberg, J.]]
+[[Category: Van Den Berg S]]
-[[Category: Wallden, K.]]
+[[Category: Wallden K]]
-[[Category: Weigelt, J.]]
+[[Category: Weigelt J]]
-[[Category: Amino-acid biosynthesis]]
-[[Category: Aspartate]]
-[[Category: Citrulline]]
-[[Category: Ligase]]
-[[Category: Sgc]]
-[[Category: Structural genomic]]
-[[Category: Structural genomics consortium]]
-[[Category: Synthase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 13:00:43 2009''