2ncd: Difference between revisions

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-{{Seed}}
-[[Image:2ncd.png|left|200px]]
-<!--
+==NCD (NON-CLARET DISJUNCTIONAL) DIMER FROM D. MELANOGASTER==
-The line below this paragraph, containing "STRUCTURE_2ncd", creates the "Structure Box" on the page.
+<StructureSection load='2ncd' size='340' side='right'caption='[[2ncd]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2ncd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. The April 2005 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Kinesin''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2005_4 10.2210/rcsb_pdb/mom_2005_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NCD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NCD FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-{{STRUCTURE_2ncd|  PDB=2ncd  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ncd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ncd OCA], [https://pdbe.org/2ncd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ncd RCSB], [https://www.ebi.ac.uk/pdbsum/2ncd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ncd ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NCD_DROME NCD_DROME] NCD is required for normal chromosomal segregation in meiosis, in females, and in early mitotic divisions of the embryo. The NCD motor activity is directed toward the microtubule's minus end.<ref>PMID:2146510</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nc/2ncd_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ncd ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Motor proteins of the kinesin superfamily transport intracellular cargo along microtubules. Although different kinesin proteins share 30-50% amino-acid identity in their motor catalytic cores, some move to the plus end of microtubules whereas others travel in the opposite direction. Crystal structures of the catalytic cores of conventional kinesin (a plus-end-directed motor involved in organelle transport) and ncd (a minus-end-directed motor involved in chromosome segregation) are nearly identical; therefore, the structural basis for their opposite directions of movement is unknown. Here we show that the ncd 'neck' made up of 13 class-specific residues next to the superfamily-conserved catalytic core, is essential for minus-end-directed motility, as mutagenesis of these neck residues reverses the direction of ncd motion. By solving the 2.5 A structure of a functional ncd dimer, we show that the ncd neck (a coiled-coil) differs from the corresponding region in the kinesin neck (an interrupted beta-strand), although both necks interact with similar elements in the catalytic cores. The distinct neck architectures also confer different symmetries to the ncd and kinesin dimers and position these motors with appropriate directional bias on the microtubule.
-===NCD (NON-CLARET DISJUNCTIONAL) DIMER FROM D. MELANOGASTER===
+Direction determination in the minus-end-directed kinesin motor ncd.,Sablin EP, Case RB, Dai SC, Hart CL, Ruby A, Vale RD, Fletterick RJ Nature. 1998 Oct 22;395(6704):813-6. PMID:9796817<ref>PMID:9796817</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2ncd" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_9796817}}, adds the Publication Abstract to the page
+*[[Kinesin 3D Structures|Kinesin 3D Structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 9796817 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_9796817}}
+__TOC__
+</StructureSection>
-==About this Structure==
-NCD is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. The April 2005 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Kinesin''  by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2005_4 10.2210/rcsb_pdb/mom_2005_4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NCD OCA].
-==Reference==
-<ref group="xtra">PMID:9796817</ref><references group="xtra"/>
 [[Category: Drosophila melanogaster]]
 [[Category: Kinesin]]
-[[Category: Case, R B.]]
+[[Category: Large Structures]]
-[[Category: Dai, S C.]]
+[[Category: RCSB PDB Molecule of the Month]]
-[[Category: Fletterick, R J.]]
+[[Category: Case RB]]
-[[Category: Hart, C L.]]
+[[Category: Dai SC]]
-[[Category: Ruby, A.]]
+[[Category: Fletterick RJ]]
-[[Category: Sablin, E P.]]
+[[Category: Hart CL]]
-[[Category: Vale, R D.]]
+[[Category: Ruby A]]
-[[Category: Kinesin]]
+[[Category: Sablin EP]]
-[[Category: Microtubule-based motor]]
+[[Category: Vale RD]]
-[[Category: Ncd]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:03:12 2009''