2bcx: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:2bcx.jpg|left|200px]]<br /><applet load="2bcx" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="2bcx, resolution 2.000&Aring;" />
-'''Crystal structure of calmodulin in complex with a ryanodine receptor peptide'''<br />
-==Overview==
+==Crystal structure of calmodulin in complex with a ryanodine receptor peptide==
-Calmodulin regulates ryanodine receptor-mediated Ca(2+) release through a, conserved binding site. The crystal structure of Ca(2+)-calmodulin bound, to this conserved site reveals that calmodulin recognizes two hydrophobic, anchor residues at a novel "1-17" spacing that brings the calmodulin lobes, close together but prevents them from contacting one another. NMR residual, dipolar couplings demonstrate that the detailed structure of each lobe is, preserved in solution but also show that the lobes experience domain, motions within the complex. FRET measurements confirm the close approach, of the lobes in binding the 1-17 target and show that calmodulin binds, with one lobe to a peptide lacking the second anchor. We suggest that, calmodulin regulates the Ca(2+) channel by switching between the, contiguous binding mode seen in our crystal structure and a state where, one lobe of calmodulin contacts the conserved binding site while the other, interacts with a noncontiguous site on the channel.
+<StructureSection load='2bcx' size='340' side='right'caption='[[2bcx]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2bcx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BCX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BCX FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bcx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bcx OCA], [https://pdbe.org/2bcx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bcx RCSB], [https://www.ebi.ac.uk/pdbsum/2bcx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bcx ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CALM_CHICK CALM_CHICK] Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bc/2bcx_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bcx ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-BCX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BCX OCA].
+*[[Calmodulin 3D structures|Calmodulin 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Complex of calmodulin with a ryanodine receptor target reveals a novel, flexible binding mode., Maximciuc AA, Putkey JA, Shamoo Y, Mackenzie KR, Structure. 2006 Oct;14(10):1547-56. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17027503 17027503]
 [[Category: Gallus gallus]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: MacKenzie, K.R.]]
+[[Category: Oryctolagus cuniculus]]
-[[Category: Maximciuc, A.A.]]
+[[Category: MacKenzie KR]]
-[[Category: Shamoo, Y.]]
+[[Category: Maximciuc AA]]
-[[Category: CA]]
+[[Category: Shamoo Y]]
-[[Category: ef-hand]]
-[[Category: type-2 turn]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:44:06 2007''