2b2g: Difference between revisions
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New page: left|200px<br /><applet load="2b2g" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b2g, resolution 3.02Å" /> '''MS2 Wild-type RNA st... |
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== | ==MS2 Wild-type RNA stemloop complexed with an N87S mutant MS2 capsid== | ||
Sequence-specific interactions between RNA stem-loops and coat protein | <StructureSection load='2b2g' size='340' side='right'caption='[[2b2g]], [[Resolution|resolution]] 3.02Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2b2g]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_MS2 Escherichia phage MS2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B2G FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.02Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2g OCA], [https://pdbe.org/2b2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b2g RCSB], [https://www.ebi.ac.uk/pdbsum/2b2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b2g ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/CAPSD_BPMS2 CAPSD_BPMS2] Self-assembles to form the T=3 icosahedral virus shell that protects the viral nucleic acid. Acts as a translational repressor by binding with high specificity to a single stem-loop structure in the genomic RNA that contains the initiation codon of the gene for the viral replicase. Involved in virus assembly through the interaction between a capsid protein dimer and the multiple packaging signals present in the RNA genome.<ref>PMID:16531233</ref> <ref>PMID:18662904</ref> <ref>PMID:26608810</ref> <ref>PMID:8254664</ref> <ref>PMID:9245600</ref> <ref>PMID:9469847</ref> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/2b2g_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b2g ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Sequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism. | |||
Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2.,Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG Structure. 2006 Mar;14(3):487-95. PMID:16531233<ref>PMID:16531233</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 2b2g" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Adams | <references/> | ||
[[Category: Baron | __TOC__ | ||
[[Category: Grahn | </StructureSection> | ||
[[Category: Helgstrand | [[Category: Escherichia phage MS2]] | ||
[[Category: Horn | [[Category: Large Structures]] | ||
[[Category: Lago | [[Category: Adams CJ]] | ||
[[Category: Liljas | [[Category: Baron AJ]] | ||
[[Category: Peabody | [[Category: Grahn E]] | ||
[[Category: Phillips | [[Category: Helgstrand C]] | ||
[[Category: Stockley | [[Category: Horn WT]] | ||
[[Category: Stonehouse | [[Category: Lago H]] | ||
[[Category: Tars | [[Category: Liljas L]] | ||
[[Category: Peabody DS]] | |||
[[Category: Phillips SEV]] | |||
[[Category: Stockley PG]] | |||
[[Category: Stonehouse NJ]] | |||
[[Category: Tars K]] | |||
Latest revision as of 11:19, 25 October 2023
MS2 Wild-type RNA stemloop complexed with an N87S mutant MS2 capsidMS2 Wild-type RNA stemloop complexed with an N87S mutant MS2 capsid
Structural highlights
FunctionCAPSD_BPMS2 Self-assembles to form the T=3 icosahedral virus shell that protects the viral nucleic acid. Acts as a translational repressor by binding with high specificity to a single stem-loop structure in the genomic RNA that contains the initiation codon of the gene for the viral replicase. Involved in virus assembly through the interaction between a capsid protein dimer and the multiple packaging signals present in the RNA genome.[1] [2] [3] [4] [5] [6] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedSequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism. Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2.,Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG Structure. 2006 Mar;14(3):487-95. PMID:16531233[7] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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