1tk7: Difference between revisions

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-{{Seed}}
-[[Image:1tk7.png|left|200px]]
-<!--
+==NMR structure of WW domains (WW3-4) from Suppressor of Deltex==
-The line below this paragraph, containing "STRUCTURE_1tk7", creates the "Structure Box" on the page.
+<StructureSection load='1tk7' size='340' side='right'caption='[[1tk7]]' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1tk7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TK7 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tk7 OCA], [https://pdbe.org/1tk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tk7 RCSB], [https://www.ebi.ac.uk/pdbsum/1tk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tk7 ProSAT]</span></td></tr>
-{{STRUCTURE_1tk7|  PDB=1tk7  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SUDX_DROME SUDX_DROME] E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Down-regulates Notch/N signaling pathway, probably by promoting Notch ubiquitination, endocytosis and degradation. Involved in wing growth and leg joint formation.<ref>PMID:12648496</ref> <ref>PMID:15620650</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tk/1tk7_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tk7 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+WW domains mediate protein recognition, usually though binding to proline-rich sequences. In many proteins, WW domains occur in tandem arrays. Whether or how individual domains within such arrays cooperate to recognize biological partners is, as yet, poorly characterized. An important question is whether functional diversity of different WW domain proteins is reflected in the structural organization and ligand interaction mechanisms of their multiple domains. We have determined the solution structure and dynamics of a pair of WW domains (WW3-4) from a Drosophila Nedd4 family protein called Suppressor of deltex (Su(dx)), a regulator of Notch receptor signaling. We find that the binding of a type 1 PPPY ligand to WW3 stabilizes the structure with effects propagating to the WW4 domain, a domain that is not active for ligand binding. Both WW domains adopt the characteristic triple-stranded beta-sheet structure, and significantly, this is the first example of a WW domain structure to include a domain (WW4) lacking the second conserved Trp (replaced by Phe). The domains are connected by a flexible linker, which allows a hinge-like motion of domains that may be important for the recognition of functionally relevant targets. Our results contrast markedly with those of the only previously determined three-dimensional structure of tandem WW domains, that of the rigidly oriented WW domain pair from the RNA-splicing factor Prp40. Our data illustrate that arrays of WW domains can exhibit a variety of higher order structures and ligand interaction mechanisms.
-===NMR structure of WW domains (WW3-4) from Suppressor of Deltex===
+The structure and dynamics of tandem WW domains in a negative regulator of notch signaling, Suppressor of deltex.,Fedoroff OY, Townson SA, Golovanov AP, Baron M, Avis JM J Biol Chem. 2004 Aug 13;279(33):34991-5000. Epub 2004 Jun 1. PMID:15173166<ref>PMID:15173166</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_15173166}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1tk7" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 15173166 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_15173166}}
+__TOC__
+</StructureSection>
-==About this Structure==
-TK7 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TK7 OCA].
-==Reference==
-<ref group="xtra">PMID:15173166</ref><references group="xtra"/>
 [[Category: Drosophila melanogaster]]
-[[Category: Avis, J M.]]
+[[Category: Large Structures]]
-[[Category: Baron, M.]]
+[[Category: Avis JM]]
-[[Category: Fedoroff, O Y.]]
+[[Category: Baron M]]
-[[Category: Golovanov, A P.]]
+[[Category: Fedoroff OY]]
-[[Category: Townson, S A.]]
+[[Category: Golovanov AP]]
-[[Category: Notch]]
+[[Category: Townson SA]]
-[[Category: Ww domain]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:38:19 2009''