1z3h: Difference between revisions

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New page: left|200px<br /><applet load="1z3h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z3h, resolution 3.100Å" /> '''The exportin Cse1 i...
 
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[[Image:1z3h.gif|left|200px]]<br /><applet load="1z3h" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1z3h, resolution 3.100&Aring;" />
'''The exportin Cse1 in its cargo-free, cytoplasmic state'''<br />


==Overview==
==The exportin Cse1 in its cargo-free, cytoplasmic state==
Cse1 mediates nuclear export of importin alpha, the nuclear localization, signal (NLS) import adaptor. We report the 3.1 A resolution structure of, cargo-free Cse1, representing this HEAT repeat protein in its cytosolic, state. Cse1 is compact, consisting of N- and C-terminal arches that, interact to form a ring. Comparison with the structure of cargo-bound Cse1, shows a major conformational change leading to opening of the structure, upon cargo binding. The largest structural changes occur within a hinge, region centered at HEAT repeat 8. This repeat contains a conserved, insertion that connects the RanGTP and importin alpha contact sites and, that is essential for binding. In the cargo-free state, the RanGTP binding, sites are occluded and the importin alpha sites are distorted. Mutations, that destabilize the N- to C-terminal interaction uncouple importin alpha, and Ran binding, suggesting that the closed conformation prevents, association with importin alpha.
<StructureSection load='1z3h' size='340' side='right'caption='[[1z3h]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1z3h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z3H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z3H FirstGlance]. <br>
1Z3H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Z3H OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3h OCA], [https://pdbe.org/1z3h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z3h RCSB], [https://www.ebi.ac.uk/pdbsum/1z3h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z3h ProSAT]</span></td></tr>
The structure of the nuclear export receptor Cse1 in its cytosolic state reveals a closed conformation incompatible with cargo binding., Cook A, Fernandez E, Lindner D, Ebert J, Schlenstedt G, Conti E, Mol Cell. 2005 Apr 29;18(3):355-67. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15866177 15866177]
</table>
== Function ==
[https://www.uniprot.org/uniprot/CSE1_YEAST CSE1_YEAST] Export receptor for importin alpha (SRP1). Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates have been released into the nucleoplasm.<ref>PMID:9744791</ref> <ref>PMID:9857050</ref> <ref>PMID:9774694</ref> <ref>PMID:10394916</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z3/1z3h_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1z3h ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Conti E]]
[[Category: Conti, E.]]
[[Category: Cook A]]
[[Category: Cook, A.]]
[[Category: Ebert J]]
[[Category: Ebert, J.]]
[[Category: Fernandez E]]
[[Category: Fernandez, E.]]
[[Category: Lindner D]]
[[Category: Lindner, D.]]
[[Category: Schlenstedt G]]
[[Category: Schlenstedt, G.]]
[[Category: MG]]
[[Category: cse1]]
[[Category: exportin]]
[[Category: heat repeat]]
[[Category: nuclear transport]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 07:13:17 2007''

Latest revision as of 16:38, 13 March 2024

The exportin Cse1 in its cargo-free, cytoplasmic stateThe exportin Cse1 in its cargo-free, cytoplasmic state

Structural highlights

1z3h is a 2 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.1Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CSE1_YEAST Export receptor for importin alpha (SRP1). Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates have been released into the nucleoplasm.[1] [2] [3] [4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Kunzler M, Hurt EC. Cse1p functions as the nuclear export receptor for importin alpha in yeast. FEBS Lett. 1998 Aug 21;433(3):185-90. PMID:9744791
  2. ↑ Hood JK, Silver PA. Cse1p is required for export of Srp1p/importin-alpha from the nucleus in Saccharomyces cerevisiae. J Biol Chem. 1998 Dec 25;273(52):35142-6. PMID:9857050
  3. ↑ Solsbacher J, Maurer P, Bischoff FR, Schlenstedt G. Cse1p is involved in export of yeast importin alpha from the nucleus. Mol Cell Biol. 1998 Nov;18(11):6805-15. PMID:9774694
  4. ↑ Schroeder AJ, Chen XH, Xiao Z, Fitzgerald-Hayes M. Genetic evidence for interactions between yeast importin alpha (Srp1p) and its nuclear export receptor, Cse1p. Mol Gen Genet. 1999 Jun;261(4-5):788-95. PMID:10394916

1z3h, resolution 3.10Å

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