1yg9: Difference between revisions
New page: left|200px<br /><applet load="1yg9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yg9, resolution 1.30Å" /> '''The structure of mut... |
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== | ==The structure of mutant (N93Q) of bla g 2== | ||
The crystal structure of Bla g 2 was solved in order to investigate the | <StructureSection load='1yg9' size='340' side='right'caption='[[1yg9]], [[Resolution|resolution]] 1.30Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1yg9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Blattella_germanica Blattella germanica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YG9 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yg9 OCA], [https://pdbe.org/1yg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yg9 RCSB], [https://www.ebi.ac.uk/pdbsum/1yg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yg9 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ASP2_BLAGE ASP2_BLAGE] Functions as a digestive enzyme in the cockroach. | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yg/1yg9_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yg9 ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The crystal structure of Bla g 2 was solved in order to investigate the structural basis for the allergenic properties of this unusual protein. This is the first structure of an aspartic protease in which conserved glycine residues, in two canonical DTG triads, are substituted by different amino acid residues. Another unprecedented feature revealed by the structure is the single phenylalanine residue insertion on the tip of the flap, with the side-chain occupying the S1 binding pocket. This and other important amino acid substitutions in the active site region of Bla g 2 modify the interactions in the vicinity of the catalytic aspartate residues, increasing the distance between them to approximately 4A and establishing unique direct contacts between the flap and the catalytic residues. We attribute the absence of substantial catalytic activity in Bla g 2 to these unusual features of the active site. Five disulfide bridges and a Zn-binding site confer stability to the protein, which may contribute to sensitization at lower levels of exposure than other allergens. | |||
Crystal structure of cockroach allergen Bla g 2, an unusual zinc binding aspartic protease with a novel mode of self-inhibition.,Gustchina A, Li M, Wunschmann S, Chapman MD, Pomes A, Wlodawer A J Mol Biol. 2005 Apr 29;348(2):433-44. PMID:15811379<ref>PMID:15811379</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1yg9" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Blattella germanica]] | [[Category: Blattella germanica]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Gustchina | [[Category: Gustchina A]] | ||
[[Category: Li | [[Category: Li M]] | ||
[[Category: Pomes | [[Category: Pomes A]] | ||
[[Category: Wlodawer | [[Category: Wlodawer A]] | ||
[[Category: Wuenschmann | [[Category: Wuenschmann S]] | ||
