1wwh: Difference between revisions

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New page: left|200px<br /><applet load="1wwh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wwh, resolution 2.70Å" /> '''Crystal structure of...
 
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[[Image:1wwh.gif|left|200px]]<br /><applet load="1wwh" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1wwh, resolution 2.70&Aring;" />
'''Crystal structure of the MPPN domain of mouse Nup35'''<br />


==Overview==
==Crystal structure of the MPPN domain of mouse Nup35==
The nuclear pore complex mediates the transport of macromolecules across, the nuclear envelope (NE). The vertebrate nuclear pore protein Nup35, the, ortholog of Saccharomyces cerevisiae Nup53p, is suggested to interact with, the NE membrane and to be required for nuclear morphology. The highly, conserved region between vertebrate Nup35 and yeast Nup53p is predicted to, contain an RNA-recognition motif (RRM) domain. Due to its low level of, sequence homology with other RRM domains, the RNP1 and RNP2 motifs have, not been identified in its primary structure. In the present study, we, solved the crystal structure of the RRM domain of mouse Nup35 at 2.7 A, resolution. The Nup35 RRM domain monomer adopts the characteristic, betaalphabetabetaalphabeta topology, as in other reported RRM domains. The, structure allowed us to locate the atypical RNP1 and RNP2 motifs. Among, the RNP motif residues, those on the beta-sheet surface are different from, those of the canonical RRM domains, while those buried in the hydrophobic, core are highly conserved. The RRM domain forms a homodimer in the, crystal, in accordance with analytical ultracentrifugation experiments., The beta-sheet surface of the RRM domain, with its atypical RNP motifs, contributes to homodimerization mainly by hydrophobic interactions: the, side-chain of Met236 in the beta4 strand of one Nup35 molecule is, sandwiched by the aromatic side-chains of Phe178 in the beta1 strand and, Trp209 in the beta3 strand of the other Nup35 molecule in the dimer. This, structure reveals a new homodimerization mode of the RRM domain.
<StructureSection load='1wwh' size='340' side='right'caption='[[1wwh]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1wwh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WWH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WWH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wwh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wwh OCA], [https://pdbe.org/1wwh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wwh RCSB], [https://www.ebi.ac.uk/pdbsum/1wwh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wwh ProSAT], [https://www.topsan.org/Proteins/RSGI/1wwh TOPSAN]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NUP35_MOUSE NUP35_MOUSE] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ww/1wwh_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wwh ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1WWH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WWH OCA].
*[[Nucleoporin 3D structures|Nucleoporin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The crystal structure of mouse Nup35 reveals atypical RNP motifs and novel homodimerization of the RRM domain., Handa N, Kukimoto-Niino M, Akasaka R, Kishishita S, Murayama K, Terada T, Inoue M, Kigawa T, Kose S, Imamoto N, Tanaka A, Hayashizaki Y, Shirouzu M, Yokoyama S, J Mol Biol. 2006 Oct 13;363(1):114-24. Epub 2006 Aug 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16962612 16962612]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Hamana H]]
[[Category: Hamana, H.]]
[[Category: Handa N]]
[[Category: Handa, N.]]
[[Category: Kukimoto M]]
[[Category: Kukimoto, M.]]
[[Category: Murayama K]]
[[Category: Murayama, K.]]
[[Category: Shirouzu M]]
[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
[[Category: Takemoto C]]
[[Category: Shirouzu, M.]]
[[Category: Terada T]]
[[Category: Takemoto, C.]]
[[Category: Uchikubo T]]
[[Category: Terada, T.]]
[[Category: Yokoyama S]]
[[Category: Uchikubo, T.]]
[[Category: Yokoyama, S.]]
[[Category: mppn]]
[[Category: riken structural genomics/proteomics initiative]]
[[Category: rsgi]]
[[Category: structural genomics]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 05:43:09 2007''

Latest revision as of 16:34, 13 March 2024

Crystal structure of the MPPN domain of mouse Nup35Crystal structure of the MPPN domain of mouse Nup35

Structural highlights

1wwh is a 4 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.7Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

NUP35_MOUSE Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1wwh, resolution 2.70Å

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