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=<font color = 'red'>A SMART Team Molecular Story</font><font color = 'black'> - Madison West High School Project of β2-Adrenergic Receptor</font>=
='''<font color = 'black'> A Physical Model of the β2-Adrenergic Receptor</font>'''=


=='''<font color = 'red'>A SMART Team Molecular Story</font><font color = 'black'> --- from the Madison West High School 2008 SMART Team</font>'''==


:Students -- Dianna Amasino, Axel Glaubitz, Susan Huang, Joy Li, Hsien-Yu Shih, Junyao Song, Esther Yoon, Xiao Zhu:


<applet load='2rh1.pdb' size='250' frame='true'  align='left' scene='Hoelzer_Sandbox/Building_our_model/7'/>
:Advisor: Basudeb Bhattacharyya  /  Assistant: Peter Vander Velden
<swf width="350" height="300">http://myweb.msoe.edu/~hoelzer/proteopedia%20test.swf</swf>
 
:Mentors: David Nelson, Ph.D. and Jim Keck, Ph.D., University of Wisconsin-Madison, Madison, WI
 
 
 
 
<applet load='2rh1.pdb' size='350' frame='true'  align='left' scene='Hoelzer_Sandbox/First_image/1'/>
<swf width="550" height="380">http://myweb.msoe.edu/~hoelzer/Madison West 2007-2008.swf</swf>




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==<font color = 'blue'>Creating the Physical Model of the β2-adrenergic receptor</font>==
==<font color = 'blue'>Creating the Physical Model of the β2-adrenergic receptor</font>==
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Virtually any image of a protein that can be created in the computer environment of RP-RasMol, can be converted into a physical model of the protein using rapid prototyping technology.   
Virtually any image of a protein that can be created in the computer environment of RP-RasMol, can be converted into a physical model of the protein using rapid prototyping technology.  To design our model of the β2-adrenergic receptor, we used the atomic coordinates for this structure as reported in the pdb file 2rh1, from the Ray Stevens laboratory at the Scripps Research Institute. 
 
--- Our model represents <scene name='Hoelzer_Sandbox/Image_1/1'>amino acids 29-230 and 263-342 </scene>. 
 
--- Starting with a cpk-colored, spacefilled representation of the protein, we simplified this image by converting it to an <scene name='Hoelzer_Sandbox/Image_2/1'>alpha-carbon backbone representation</scene>.  We colored the seven trans-membrane alpha helices green --- connected by loops that we colored gray.   


To design our model of the β2-adrenergic receptor, we used the atomic coordinates for this structure as reported in the pdb file 2rh1, from the Ray Stevens laboratory at the Scripps Research Institute.  * Our model represents <scene name='Hoelzer_Sandbox/Image_1/1'>amino acids 29-230 and 263-342 </scene>.  * Starting with a cpk-colored, spacefilled representation of the protein, we simplified this image by converting it to an <scene name='Hoelzer_Sandbox/Image_2/1'>alpha-carbon backbone representation</scene>.  We colored the seven trans-membrane alpha helices green --- connected by loops that we colored gray.  We then displayed four sidechains <scene name='Hoelzer_Sandbox/Spacefilled_4/7'>(Phe 193, Trp 286, Phe 289, and Phe 290)</scene> involved in the binding of a beta-blocker, and colored them blue.  The beta-blocker, <scene name='Hoelzer_Sandbox/Spacefilled_4/6'>Carazolol</scene>, was then added in a ball-and-stick format, colored orange.  The <scene name='Hoelzer_Sandbox/Spacefilled_4/8'>three cholesterol molecules </scene> resolved in this structure, bound to the outside surface of the protein, were added and displayed in a ball-and-stick format, colored red.  Finally, <scene name='Hoelzer_Sandbox/Spacefilled_4/9'>the N-terminal end </scene>of the protein was colored blue, and <scene name='Hoelzer_Sandbox/Spacefilled_4/10'>the C-terminal end </scene>was colored magenta.
--- We then displayed four sidechains <scene name='Hoelzer_Sandbox/Spacefilled_4/7'>(Phe 193, Trp 286, Phe 289, and Phe 290)</scene> involved in the binding of a beta-blocker, and colored them blue.   
 
--- The beta-blocker, <scene name='Hoelzer_Sandbox/Spacefilled_4/6'>Carazolol</scene>, was then added in a ball-and-stick format, colored orange.   
 
--- The <scene name='Hoelzer_Sandbox/Spacefilled_4/8'>three cholesterol molecules </scene> resolved in this structure, bound to the outside surface of the protein, were added and displayed in a ball-and-stick format, colored red.   
 
--- Finally, <scene name='Hoelzer_Sandbox/Spacefilled_4/9'>the N-terminal end </scene>of the protein was colored blue, and <scene name='Hoelzer_Sandbox/Spacefilled_4/10'>the C-terminal end </scene>was colored magenta.


A ply file describing this final structure was exported from RP-RasMol and sent to the MSOE Center for BioMolecular Modeling, where it was constructed from plaster powder, using a color ZCorp 3D printer.   
A ply file describing this final structure was exported from RP-RasMol and sent to the MSOE Center for BioMolecular Modeling, where it was constructed from plaster powder, using a color ZCorp 3D printer.   
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==<font color = 'blue'>References</font>==
1) Rasmussen, S.G.F., Choi, H.-J., Rosenbaum, D.M., Kobilka, T.S., Thian, F.S., Edwards, P.C., Burghammer, M., Ratnala, V.R.P., Sanishvili, R., Fischetti, R.F., Schertler, G.F.X., Weis, W.I. & Kobilka, B.K. (2007). “Crystal structure of the human [bgr]2 adrenergic G-protein-coupled receptor” Nature 450, 383-387.
2) Cherezov, V., Rosenbaum, D.M., Hanson, M.A., Rasmussen, S.G.F., Thian, F.S., Kobilka, T.S., Choi, H.-J., Kuhn, P., Weis, W.I., Kobilka, B.K. & Stevens, R.C. (2007). “High-Resolution Crystal Structure of an Engineered Human 2-Adrenergic G Protein Coupled Receptor” Science 318, 1258-1265.
3) Rosenbaum, D.M., Cherezov, V., Hanson, M.A., Rasmussen, S.G., Thian, F.S., Kobilka, T.S., Choi, H.J., Yao, X.J., Weis, W.I., Stevens, R.C. & Kobilka, B.K. (2007). “GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function” Science 318, 1266-1273.
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==<font color = 'blue'>Our Poster and Presentations</font>==
==<font color = 'blue'>Our Poster and Presentations</font>==
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We were able to present our project, poster, and our physical models at the 2008 ASBMB meeting in San Diego, CA.  The poster was presented several other times including at the Medical College of Wisconsin and at the Scripps Research Institute.  During our visit to Scripps, we met David Goodsell and Art Olsen, two molecular illustrators for the Molecular Graphics Laboratory at the institute.
We were able to present our project, poster, and our physical models at the 2008 ASBMB meeting in San Diego, CA.  The poster was presented at the Medical College of Wisconsin and at the Scripps Research Institute.  During our visit to Scripps, we met Art Olson and David Goodsell --- from the Molecular Graphics Laboratory at TSRI.


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==<font color = 'red'>MSOE Center for BioMolecular Modeling and SMART Teams</font>==
==<font color = 'red'>MSOE Center for BioMolecular Modeling and SMART Teams</font>==
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[[Image:Smart Teams photo 5.jpg|right|120px]]
[[Image:Smart Teams photo 5.jpg|right|120px]]


<font color = 'red'>SMART  Teams (S</font>tudents <font color = 'red'>M</font>odeling <font color = 'red'>A</font> <font color = 'red'>R</font>esearch <font color = 'red'>T</font>opic)is a science outreach program developed by the MSOE Center for BioMolecular Modeling.  In this program, teams of high school students work with a local resarch lab to design and build a physical model of a protein that is being investigated by the lab.  The goal of the SMART Team program is to introduce students to the real world of scince --- as it exists in a local research lab.  This developement of this program was supported by grants from the NIH-NCRR SEPA program (Science Education Partnership Award) and an HHMI Precollege Science Education Award.  For more information about this program, visit the CBM web site at www.rpc.msoe.edu/cbm.
<font color = 'red'>SMART  Teams (S</font>tudents <font color = 'red'>M</font>odeling <font color = 'red'>A</font> <font color = 'red'>R</font>esearch <font color = 'red'>T</font>opic) is a science outreach program developed by the MSOE Center for BioMolecular Modeling.  In this program, teams of high school students work with a local resarch lab to design and build a physical model of a protein that is being investigated by the lab.  The goal of the SMART Team program is to introduce students to the real world of science --- as it exists in a local research lab.  The development of this program was supported by grants from the NIH-NCRR SEPA program (Science Education Partnership Award) and an HHMI Precollege Science Education Award.  For more information about this program, visit the CBM web site at [http://www.rpc.msoe.edu/cbm www.rpc.msoe.edu/cbm] .

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Mark Hoelzer, Tim Herman
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