2w3n: Difference between revisions

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-{{Seed}}
-[[Image:2w3n.jpg|left|200px]]
-<!--
+==Structure and inhibition of the CO2-sensing carbonic anhydrase Can2 from the pathogenic fungus Cryptococcus neoformans==
-The line below this paragraph, containing "STRUCTURE_2w3n", creates the "Structure Box" on the page.
+<StructureSection load='2w3n' size='340' side='right'caption='[[2w3n]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2w3n]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryptococcus_neoformans Cryptococcus neoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W3N FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-{{STRUCTURE_2w3n|  PDB=2w3n  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w3n OCA], [https://pdbe.org/2w3n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w3n RCSB], [https://www.ebi.ac.uk/pdbsum/2w3n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w3n ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q3I4V7_CRYNV Q3I4V7_CRYNV]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w3/2w3n_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w3n ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+In the pathogenic fungus Cryptococcus neoformans, a CO(2)-sensing system is essential for survival in the natural environment ( approximately 0.03% CO(2)) and mediates the switch to virulent growth in the human host ( approximately 5% CO(2)). This system is composed of the carbonic anhydrase (CA) Can2, which catalyzes formation of bicarbonate, and the fungal, bicarbonate-stimulated adenylyl cyclase Cac1. The critical role of these enzymes for fungal metabolism and pathogenesis identifies them as targets for antifungal drugs. Here, we prove functional similarity of Can2 to the CA Nce103 from Candida albicans and describe its biochemical and structural characterization. The crystal structure of Can2 reveals that the enzyme belongs to the "plant-type" beta-CAs but carries a unique N-terminal extension that can interact with the active-site entrance of the dimer. We further tested a panel of compounds, identifying nanomolar Can2 inhibitors, and present the structure of a Can2 complex with the inhibitor and product analog acetate, revealing insights into interactions with physiological ligands and inhibitors.
-===STRUCTURE AND INHIBITION OF THE CO2-SENSING CARBONIC ANHYDRASE CAN2 FROM THE PATHOGENIC FUNGUS CRYPTOCOCCUS NEOFORMANS===
+Structure and Inhibition of the CO(2)-Sensing Carbonic Anhydrase Can2 from the Pathogenic Fungus Cryptococcus neoformans.,Schlicker C, Hall RA, Vullo D, Middelhaufe S, Gertz M, Supuran CT, Muhlschlegel FA, Steegborn C J Mol Biol. 2008 Nov 27. PMID:19071134<ref>PMID:19071134</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2w3n" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_19071134}}, adds the Publication Abstract to the page
+*[[Carbonic anhydrase 3D structures|Carbonic anhydrase 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 19071134 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_19071134}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Cryptococcus neoformans]]
-W3N is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Filobasidiella_neoformans Filobasidiella neoformans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W3N OCA].
+[[Category: Large Structures]]
+[[Category: Gertz M]]
-==Reference==
+[[Category: Hall RA]]
-Structure and Inhibition of the CO(2)-Sensing Carbonic Anhydrase Can2 from the Pathogenic Fungus Cryptococcus neoformans., Schlicker C, Hall RA, Vullo D, Middelhaufe S, Gertz M, Supuran CT, Muhlschlegel FA, Steegborn C, J Mol Biol. 2008 Nov 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/19071134 19071134]
+[[Category: Middelhaufe S]]
-[[Category: Carbonate dehydratase]]
+[[Category: Muehlschlegel FA]]
-[[Category: Filobasidiella neoformans]]
+[[Category: Schlicker C]]
-[[Category: Gertz, M.]]
+[[Category: Steegborn C]]
-[[Category: Hall, R A.]]
+[[Category: Supuran CT]]
-[[Category: Middelhaufe, S.]]
+[[Category: Vullo D]]
-[[Category: Muehlschlegel, F A.]]
-[[Category: Schlicker, C.]]
-[[Category: Steegborn, C.]]
-[[Category: Supuran, C T.]]
-[[Category: Vullo, D.]]
-[[Category: Beta-class carbonic anhydrase]]
-[[Category: Cryptococcus neoforman]]
-[[Category: Inhibition]]
-[[Category: Lyase]]
-[[Category: Sulfonamide]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 31 10:09:56 2008''