3cck: Difference between revisions

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-{{Seed}}
-[[Image:3cck.png|left|200px]]
-<!--
+==Human CD69==
-The line below this paragraph, containing "STRUCTURE_3cck", creates the "Structure Box" on the page.
+<StructureSection load='3cck' size='340' side='right'caption='[[3cck]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3cck]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CCK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CCK FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-{{STRUCTURE_3cck|  PDB=3cck  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cck FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cck OCA], [https://pdbe.org/3cck PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cck RCSB], [https://www.ebi.ac.uk/pdbsum/3cck PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cck ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CD69_HUMAN CD69_HUMAN] Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We investigated the soluble forms of the earliest activation antigen of human leukocyte CD69. This receptor is expressed at the cell surface as a type II homodimeric membrane protein. However, the elements necessary to prepare the soluble recombinant CD69 suitable for structural studies are a matter of controversy. We describe the physical, biochemical and in vivo characteristics of a highly stable soluble form of CD69 obtained by bacterial expression of an appropriate extracellular segment of this protein. Our construct has been derived from one used for CD69 crystallization by further optimization with regard to protein stability, solubility and easy crystallization under conditions promoting ligand binding. The resulting protein is stable at acidic pH and at temperatures of up to 65 degrees C, as revealed by long-term stability tests and thermal denaturation experiments. Protein NMR and crystallography confirmed the expected protein fold, and revealed additional details of the protein characteristics in solution. The soluble CD69 refolded in a form of noncovalent dimers, as revealed by gel filtration, sedimentation velocity measurements, NMR and dynamic light scattering. The soluble CD69 proved to be remarkably stable in vivo when injected into the bloodstream of experimental mice. More than 70% of the most stable CD69 proteins is preserved intact in the blood 24 h after injection, whereas the less stable CD69 variants are rapidly taken up by the liver.
-===Human CD69===
+Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice.,Vanek O, Nalezkova M, Kavan D, Borovickova I, Pompach P, Novak P, Kumar V, Vannucci L, Hudecek J, Hofbauerova K, Kopecky V Jr, Brynda J, Kolenko P, Dohnalek J, Kaderavek P, Chmelik J, Gorcik L, Zidek L, Sklenar V, Bezouska K FEBS J. 2008 Nov;275(22):5589-606. PMID:18959746<ref>PMID:18959746</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3cck" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_18959746}}, adds the Publication Abstract to the page
+*[[CD69|CD69]]
-(as it appears on PubMed at http://www.pubmed.gov), where 18959746 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_18959746}}
+__TOC__
+</StructureSection>
-==About this Structure==
-CCK is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CCK OCA].
-==Reference==
-Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice., Vanek O, Nalezkova M, Kavan D, Borovickova I, Pompach P, Novak P, Kumar V, Vannucci L, Hudecek J, Hofbauerova K, Kopecky V Jr, Brynda J, Kolenko P, Dohnalek J, Kaderavek P, Chmelik J, Gorcik L, Zidek L, Sklenar V, Bezouska K, FEBS J. 2008 Nov;275(22):5589-606. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18959746 18959746]
 [[Category: Homo sapiens]]
-[[Category: Brynda, J.]]
+[[Category: Large Structures]]
-[[Category: Rezacova, P.]]
+[[Category: Brynda J]]
-[[Category: Vanek, O.]]
+[[Category: Rezacova P]]
-[[Category: C-type lectin]]
+[[Category: Vanek O]]
-[[Category: Glycoprotein]]
-[[Category: Immune system]]
-[[Category: Leukocyte activation]]
-[[Category: Ligand binding]]
-[[Category: Membrane]]
-[[Category: Phosphoprotein]]
-[[Category: Refolding]]
-[[Category: Signal-anchor]]
-[[Category: Stability]]
-[[Category: Transmembrane]]
-[[Category: Tumor therapy]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Dec  5 09:55:10 2008''