Alpha-1-antitrypsin: Difference between revisions

{{STRUCTURE_1atu |  PDB=1atu1ezxwd.pdb  |  SCENE=User:Daniel_Seeman/Alpha-1-antitrypsin/437437437437/1}}

'''Alpha-1-antitrypsin''' (also known as α1-antitrypsin or A1AT) is an inhibitor of [[Elastase]] and [[Trypsin]].  It is a member of the '''Ser'''ine '''P'''rotease '''I'''nhibitor ([[:Category:Serpin|Serpin]]) family, and as such undergoes a conformational change where the substrate protein associates with a loop region on A1AT causing that loop to become ordered as a Beta Strand<ref name="nature_paper">''Nature'' '''455''', 1189-1190 (30 October 2008)</ref>.  In this case Trypsin (the substrate) is inhibited when a covalent bond is formed to A1AT through the newly formed Beta region<ref name="nature_paper" />.  Once bound covalently to its substrate the stability of the A1AT complex goes up drastically, making it an effective "molecular mousetrap"<ref name="nature_paper" />.  With A1AT, as with most members of the Serpin family, the transition from inactive precursor protein to active complex comes after a cleavage event<ref name="nature_paper" />.  Shown <scene name='User:Daniel_Seeman/Alpha-1-antitrypsin/437437437437/1'>on the right</scene> is a morph, generated by the <span class="plainlinks">[http://molmovdb.mbb.yale.edu/molmovdb/morph/ Yale Morph Server]</span> that shows A1AT going from its inactive form, to the conformation in which it is bound to Trypsin (also shown in the same animation)<ref>The <span class="plainlinks">[http://molmovdb.mbb.yale.edu/molmovdb/morph/ Yale Morph Server]</span></ref>.  

A mutation in the DNA coding for A1AT known as the "Z-antitrypsin mutation" can lead to formation of an inactive polymer made up of subunits of this protein.  This polymer builds up in liver cell endoplasmic reticulums leading to cirrhosis of the liver and emphysema.  The mutation is believed to decrease the flexibility of A1AT preventing it from inserting its loop region into its own stretch of beta sheet.  Instead it is suggested that the loop region of one subunit of A1AT inserts into the beta region of another subunit, leading to polymerization<ref name="nature_paper" />.

=== See Also ===

*[[1atu]]