1u0f: Difference between revisions

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-[[Image:1u0f.jpg|left|200px]]<br /><applet load="1u0f" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1u0f, resolution 1.60&Aring;" />
-'''Crystal structure of mouse phosphoglucose isomerase in complex with glucose 6-phosphate'''<br />
-==Overview==
+==Crystal structure of mouse phosphoglucose isomerase in complex with glucose 6-phosphate==
-Phosphoglucose isomerase (PGI) is an enzyme of glycolysis that, interconverts glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P), but, outside the cell, is a multifunctional cytokine. High-resolution, crystal structures of the enzyme from mouse have been determined in native, form and in complex with the inhibitor erythrose 4-phosphate, and with the, substrate glucose 6-phosphate. In the substrate-bound structure, the, glucose sugar is observed in both straight-chain and ring forms. This, structure supports a specific role for Lys518 in enzyme-catalyzed ring, opening and we present a "push-pull" mechanism in which His388 breaks the, O5-C1 bond by donating a proton to the ring oxygen atom and, simultaneously, Lys518 abstracts a proton from the C1 hydroxyl group. The, reverse occurs in ring closure. The transition from ring form to, straight-chain substrate is achieved through rotation of the C3-C4 bond, which brings the C1-C2 region into close proximity to Glu357, the base, catalyst for the isomerization step. The structure with G6P also explains, the specificity of PGI for glucose 6-phosphate over mannose 6-isomerase, (M6P). To isomerize M6P to F6P requires a rotation of its C2-C3 bond but, in PGI this is sterically blocked by Gln511.
+<StructureSection load='1u0f' size='340' side='right'caption='[[1u0f]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1u0f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U0F FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=G6P:ALPHA-D-GLUCOSE-6-PHOSPHATE'>G6P</scene>, <scene name='pdbligand=G6Q:GLUCOSE-6-PHOSPHATE'>G6Q</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0f OCA], [https://pdbe.org/1u0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u0f RCSB], [https://www.ebi.ac.uk/pdbsum/1u0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0f ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/G6PI_MOUSE G6PI_MOUSE] Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u0/1u0f_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u0f ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-U0F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with G6P, G6Q, SO4, BME and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U0F OCA].
+*[[Phosphoglucose isomerase 3D structures|Phosphoglucose isomerase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-The crystal structure of mouse phosphoglucose isomerase at 1.6A resolution and its complex with glucose 6-phosphate reveals the catalytic mechanism of sugar ring opening., Graham Solomons JT, Zimmerly EM, Burns S, Krishnamurthy N, Swan MK, Krings S, Muirhead H, Chirgwin J, Davies C, J Mol Biol. 2004 Sep 17;342(3):847-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15342241 15342241]
+[[Category: Large Structures]]
-[[Category: Glucose-6-phosphate isomerase]]
 [[Category: Mus musculus]]
-[[Category: Single protein]]
+[[Category: Burns S]]
-[[Category: Burns, S.]]
+[[Category: Chirgwin J]]
-[[Category: Chirgwin, J.]]
+[[Category: Davies C]]
-[[Category: Davies, C.]]
+[[Category: Krings S]]
-[[Category: Krings, S.]]
+[[Category: Krishnamurthy N]]
-[[Category: Krishnamurthy, N.]]
+[[Category: Muirhead H]]
-[[Category: Muirhead, H.]]
+[[Category: Solomons JTG]]
-[[Category: Solomons, J.T.G.]]
+[[Category: Swan MK]]
-[[Category: Swan, M.K.]]
+[[Category: Zimmerly EM]]
-[[Category: Zimmerly, E.M.]]
-[[Category: BME]]
-[[Category: G6P]]
-[[Category: G6Q]]
-[[Category: GOL]]
-[[Category: SO4]]
-[[Category: aldose-ketose isomerase]]
-[[Category: dimer]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:44:47 2007''