1tyq: Difference between revisions

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-[[Image:1tyq.jpg|left|200px]]<br /><applet load="1tyq" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1tyq, resolution 2.55&Aring;" />
-'''Crystal structure of Arp2/3 complex with bound ATP and calcium'''<br />
-==Overview==
+==Crystal structure of Arp2/3 complex with bound ATP and calcium==
-Actin-related protein (Arp) 2/3 complex stimulates formation of actin, filaments at the leading edge of motile cells. Nucleation of filaments, depends on hydrolysis of ATP bound to Arp2. Here we report crystal, structures of Arp2/3 complex with bound ATP or ADP. The nucleotides are, immobilized on the face of subdomains 3 and 4 of Arp2, whereas subdomains, 1 and 2 are flexible and absent from the electron density maps. This, flexibility may explain why Arp2 does not hydrolyze ATP until the complex, is activated. ATP stabilizes a relatively closed conformation of Arp3 with, the gamma-phosphate bridging loops from opposite sides of the cleft. ADP, binds Arp3 in a unique conformation that favors an open cleft, revealing a, conformational change that may occur in actin and Arps when ATP is, hydrolyzed and phosphate dissociates. These structures provide the an, opportunity to compare all nucleotide-binding states in an actin-related, protein and give insights into the function of both the Arp2/3 complex and, actin.
+<StructureSection load='1tyq' size='340' side='right'caption='[[1tyq]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1tyq]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TYQ FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tyq OCA], [https://pdbe.org/1tyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tyq RCSB], [https://www.ebi.ac.uk/pdbsum/1tyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tyq ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ARP3_BOVIN ARP3_BOVIN] Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. Plays a role in ciliogenesis (By similarity).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ty/1tyq_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tyq ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Actin-related protein (Arp) 2/3 complex stimulates formation of actin filaments at the leading edge of motile cells. Nucleation of filaments depends on hydrolysis of ATP bound to Arp2. Here we report crystal structures of Arp2/3 complex with bound ATP or ADP. The nucleotides are immobilized on the face of subdomains 3 and 4 of Arp2, whereas subdomains 1 and 2 are flexible and absent from the electron density maps. This flexibility may explain why Arp2 does not hydrolyze ATP until the complex is activated. ATP stabilizes a relatively closed conformation of Arp3 with the gamma-phosphate bridging loops from opposite sides of the cleft. ADP binds Arp3 in a unique conformation that favors an open cleft, revealing a conformational change that may occur in actin and Arps when ATP is hydrolyzed and phosphate dissociates. These structures provide the an opportunity to compare all nucleotide-binding states in an actin-related protein and give insights into the function of both the Arp2/3 complex and actin.
-==About this Structure==
+Crystal structures of actin-related protein 2/3 complex with bound ATP or ADP.,Nolen BJ, Littlefield RS, Pollard TD Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15627-32. Epub 2004 Oct 25. PMID:15505213<ref>PMID:15505213</ref>
-TYQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with CA and ATP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TYQ OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-Crystal structures of actin-related protein 2/3 complex with bound ATP or ADP., Nolen BJ, Littlefield RS, Pollard TD, Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15627-32. Epub 2004 Oct 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15505213 15505213]
+</div>
+<div class="pdbe-citations 1tyq" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Actin-related protein 3D structures|Actin-related protein 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Bos taurus]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Littlefield, R.S.]]
+[[Category: Littlefield RS]]
-[[Category: Nolen, B.J.]]
+[[Category: Nolen BJ]]
-[[Category: Pollard, T.D.]]
+[[Category: Pollard TD]]
-[[Category: ATP]]
-[[Category: CA]]
-[[Category: structural protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:42:09 2007''