2v4e: Difference between revisions

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-{{Seed}}
-[[Image:2v4e.jpg|left|200px]]
-<!--
+==A non-cytotoxic DsRed variant for whole-cell labeling==
-The line below this paragraph, containing "STRUCTURE_2v4e", creates the "Structure Box" on the page.
+<StructureSection load='2v4e' size='340' side='right'caption='[[2v4e]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2v4e]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V4E FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr>
-{{STRUCTURE_2v4e|  PDB=2v4e  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v4e OCA], [https://pdbe.org/2v4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v4e RCSB], [https://www.ebi.ac.uk/pdbsum/2v4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v4e ProSAT]</span></td></tr>
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v4/2v4e_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v4e ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.
-===A NON-CYTOTOXIC DSRED VARIANT FOR WHOLE-CELL LABELING===
+A noncytotoxic DsRed variant for whole-cell labeling.,Strack RL, Strongin DE, Bhattacharyya D, Tao W, Berman A, Broxmeyer HE, Keenan RJ, Glick BS Nat Methods. 2008 Nov;5(11):955-7. Epub 2008 Oct 26. PMID:18953349<ref>PMID:18953349</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2v4e" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_18953349}}, adds the Publication Abstract to the page
+*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 18953349 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_18953349}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Discosoma sp]]
-V4E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Discosoma_sp. Discosoma sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V4E OCA].
+[[Category: Large Structures]]
+[[Category: Berman A]]
-==Reference==
+[[Category: Bhattacharyya D]]
-A noncytotoxic DsRed variant for whole-cell labeling., Strack RL, Strongin DE, Bhattacharyya D, Tao W, Berman A, Broxmeyer HE, Keenan RJ, Glick BS, Nat Methods. 2008 Nov;5(11):955-7. Epub 2008 Oct 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18953349 18953349]
+[[Category: Broxmeyer HE]]
-[[Category: Discosoma sp.]]
+[[Category: Glick BS]]
-[[Category: Single protein]]
+[[Category: Keenan RJ]]
-[[Category: Berman, A.]]
+[[Category: Strack RL]]
-[[Category: Bhattacharyya, D.]]
+[[Category: Strongin DE]]
-[[Category: Broxmeyer, H E.]]
+[[Category: Tao W]]
-[[Category: Glick, B S.]]
-[[Category: Keenan, R J.]]
-[[Category: Strack, R L.]]
-[[Category: Strongin, D E.]]
-[[Category: Tao, W.]]
-[[Category: Chromophore]]
-[[Category: Dsred]]
-[[Category: Fluorescent protein]]
-[[Category: Luminescence]]
-[[Category: Photoprotein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov  5 12:24:32 2008''