3exd: Difference between revisions

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[[Image:3exd.jpg|left|200px]]


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==Sulfur-SAD phased HEWL Crystal==
The line below this paragraph, containing "STRUCTURE_3exd", creates the "Structure Box" on the page.
<StructureSection load='3exd' size='340' side='right'caption='[[3exd]], [[Resolution|resolution]] 1.49&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3exd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EXD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EXD FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.49&#8491;</td></tr>
-->
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3exd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3exd OCA], [https://pdbe.org/3exd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3exd RCSB], [https://www.ebi.ac.uk/pdbsum/3exd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3exd ProSAT]</span></td></tr>
{{STRUCTURE_3exd|  PDB=3exd  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/3exd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3exd ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Brazilian Synchrotron Light Laboratory [Laboratorio Nacional de Luz Sincrotron (LNLS), Campinas, SP, Brazil] is the first commissioned synchrotron light source in the southern hemisphere. The first wiggler macromolecular crystallography beamline (MX2) at the LNLS has been recently constructed and brought into operation. Here the technical design, experimental set-up, parameters of the beamline and the first experimental results obtained at MX2 are described. The beamline operates on a 2.0 T hybrid 30-pole wiggler, and its optical layout includes collimating mirror, Si(111) double-crystal monochromator and toroidal bendable mirror. The measured flux density at the sample position at 8.7 eV reaches 4.8 x 10(11) photons s(-1) mm(-2) (100 mA)(-1). The beamline is equipped with a MarResearch Desktop Beamline Goniostat (MarDTB) and 3 x 3 MarMosaic225 CCD detector, and is controlled by a customized version of the Blu-Ice software. A description of the first X-ray diffraction data sets collected at the MX2 LNLS beamline and used for macromolecular crystal structure solution is also provided.


===Sulfur-SAD phased HEWL Crystal===
The MX2 macromolecular crystallography beamline: a wiggler X-ray source at the LNLS.,Guimaraes BG, Sanfelici L, Neuenschwander RT, Rodrigues F, Grizolli WC, Raulik MA, Piton JR, Meyer BC, Nascimento AS, Polikarpov I J Synchrotron Radiat. 2009 Jan;16(Pt 1):69-75. Epub 2008 Nov 27. PMID:19096177<ref>PMID:19096177</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3exd" style="background-color:#fffaf0;"></div>


==About this Structure==
==See Also==
3EXD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EXD OCA].
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
[[Category: Lysozyme]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Liberato MV]]
[[Category: Liberato, M V.]]
[[Category: Nascimento AS]]
[[Category: Nascimento, A S.]]
[[Category: Polikarpov I]]
[[Category: Polikarpov, I.]]
[[Category: Allergen]]
[[Category: Antimicrobial]]
[[Category: Bacteriolytic enzyme]]
[[Category: Glycosidase]]
[[Category: Hewl]]
[[Category: Hydrolase]]
[[Category: Sad phasing]]
[[Category: Sulfur]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 29 11:04:39 2008''

Latest revision as of 12:05, 30 October 2024

Sulfur-SAD phased HEWL CrystalSulfur-SAD phased HEWL Crystal

Structural highlights

3exd is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.49Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The Brazilian Synchrotron Light Laboratory [Laboratorio Nacional de Luz Sincrotron (LNLS), Campinas, SP, Brazil] is the first commissioned synchrotron light source in the southern hemisphere. The first wiggler macromolecular crystallography beamline (MX2) at the LNLS has been recently constructed and brought into operation. Here the technical design, experimental set-up, parameters of the beamline and the first experimental results obtained at MX2 are described. The beamline operates on a 2.0 T hybrid 30-pole wiggler, and its optical layout includes collimating mirror, Si(111) double-crystal monochromator and toroidal bendable mirror. The measured flux density at the sample position at 8.7 eV reaches 4.8 x 10(11) photons s(-1) mm(-2) (100 mA)(-1). The beamline is equipped with a MarResearch Desktop Beamline Goniostat (MarDTB) and 3 x 3 MarMosaic225 CCD detector, and is controlled by a customized version of the Blu-Ice software. A description of the first X-ray diffraction data sets collected at the MX2 LNLS beamline and used for macromolecular crystal structure solution is also provided.

The MX2 macromolecular crystallography beamline: a wiggler X-ray source at the LNLS.,Guimaraes BG, Sanfelici L, Neuenschwander RT, Rodrigues F, Grizolli WC, Raulik MA, Piton JR, Meyer BC, Nascimento AS, Polikarpov I J Synchrotron Radiat. 2009 Jan;16(Pt 1):69-75. Epub 2008 Nov 27. PMID:19096177[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
  2. ↑ Guimaraes BG, Sanfelici L, Neuenschwander RT, Rodrigues F, Grizolli WC, Raulik MA, Piton JR, Meyer BC, Nascimento AS, Polikarpov I. The MX2 macromolecular crystallography beamline: a wiggler X-ray source at the LNLS. J Synchrotron Radiat. 2009 Jan;16(Pt 1):69-75. Epub 2008 Nov 27. PMID:19096177 doi:10.1107/S0909049508034870

3exd, resolution 1.49Å

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