3et6: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:3et6.jpg|left|200px]]
-<!--
+==The crystal structure of the catalytic domain of a eukaryotic guanylate cyclase==
-The line below this paragraph, containing "STRUCTURE_3et6", creates the "Structure Box" on the page.
+<StructureSection load='3et6' size='340' side='right'caption='[[3et6]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3et6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. The January 2011 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Nitric Oxide Synthase''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2011_1 10.2210/rcsb_pdb/mom_2011_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ET6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ET6 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAS:S-(DIMETHYLARSENIC)CYSTEINE'>CAS</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-{{STRUCTURE_3et6|  PDB=3et6  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3et6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3et6 OCA], [https://pdbe.org/3et6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3et6 RCSB], [https://www.ebi.ac.uk/pdbsum/3et6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3et6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q5YLC2_CHLRE Q5YLC2_CHLRE]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/et/3et6_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3et6 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+BACKGROUND: Soluble guanylate cyclases generate cyclic GMP when bound to nitric oxide, thereby linking nitric oxide levels to the control of processes such as vascular homeostasis and neurotransmission. The guanylate cyclase catalytic module, for which no structure has been determined at present, is a class III nucleotide cyclase domain that is also found in mammalian membrane-bound guanylate and adenylate cyclases. RESULTS: We have determined the crystal structure of the catalytic domain of a soluble guanylate cyclase from the green algae Chlamydomonas reinhardtii at 2.55 A resolution, and show that it is a dimeric molecule. CONCLUSION: Comparison of the structure of the guanylate cyclase domain with the known structures of adenylate cyclases confirms the close similarity in architecture between these two enzymes, as expected from their sequence similarity. The comparison also suggests that the crystallized guanylate cyclase is in an inactive conformation, and the structure provides indications as to how activation might occur. We demonstrate that the two active sites in the dimer exhibit positive cooperativity, with a Hill coefficient of approximately 1.5. Positive cooperativity has also been observed in the homodimeric mammalian membrane-bound guanylate cyclases. The structure described here provides a reliable model for functional analysis of mammalian guanylate cyclases, which are closely related in sequence.
-===The crystal structure of the catalytic domain of a eukaryotic guanylate cyclase===
+The crystal structure of the catalytic domain of a eukaryotic guanylate cyclase.,Winger JA, Derbyshire ER, Lamers MH, Marletta MA, Kuriyan J BMC Struct Biol. 2008 Oct 7;8:42. PMID:18842118<ref>PMID:18842118</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3et6" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-ET6 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ET6 OCA].
+*[[Guanylate cyclase 3D structures|Guanylate cyclase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Chlamydomonas reinhardtii]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Derbyshire, E R.]]
+[[Category: Nitric Oxide Synthase]]
-[[Category: Kuriyan, J.]]
+[[Category: RCSB PDB Molecule of the Month]]
-[[Category: Lamers, M H.]]
+[[Category: Derbyshire ER]]
-[[Category: Marletta, M A.]]
+[[Category: Kuriyan J]]
-[[Category: Winger, J A.]]
+[[Category: Lamers MH]]
-[[Category: Dimethylarsenic]]
+[[Category: Marletta MA]]
-[[Category: Guanylate cyclase]]
+[[Category: Winger JA]]
-[[Category: Guanylyl cyclase]]
-[[Category: Lyase]]
-[[Category: Membrane]]
-[[Category: Transmembrane]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 15 15:46:34 2008''