1t1b: Difference between revisions

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New page: left|200px<br /><applet load="1t1b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t1b, resolution 1.60Å" /> '''Late intermediate IL...
 
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[[Image:1t1b.gif|left|200px]]<br /><applet load="1t1b" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1t1b, resolution 1.60&Aring;" />
'''Late intermediate IL2 from time-resolved crystallography of the E46Q mutant of PYP'''<br />


==Overview==
==Late intermediate IL2 from time-resolved crystallography of the E46Q mutant of PYP==
In the bacterial photoreceptor photoactive yellow protein (PYP), absorption of blue light by its chromophore leads to a conformational, change in the protein associated with differential signaling activity, as, it executes a reversible photocycle. Time-resolved Laue crystallography, allows structural snapshots (as short as 150 ps) of high crystallographic, resolution (approximately 1.6 A) to be taken of a protein as it functions., Here, we analyze by singular value decomposition a comprehensive, time-resolved crystallographic data set of the E46Q mutant of PYP, throughout the photocycle spanning 10 ns-100 ms. We identify and refine, the structures of five distinct intermediates and provide a plausible, chemical kinetic mechanism for their inter conversion. A clear structural, progression is visible in these intermediates, in which a signal generated, at the chromophore propagates through a distinct structural pathway of, conserved residues and results in structural changes near the N terminus, over 20 A distant from the chromophore.
<StructureSection load='1t1b' size='340' side='right'caption='[[1t1b]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1t1b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T1B FirstGlance]. <br>
1T1B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila] with HC4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T1B OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HC4:4-HYDROXYCINNAMIC+ACID'>HC4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1b OCA], [https://pdbe.org/1t1b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t1b RCSB], [https://www.ebi.ac.uk/pdbsum/1t1b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t1b ProSAT]</span></td></tr>
A structural pathway for signaling in the E46Q mutant of photoactive yellow protein., Rajagopal S, Anderson S, Srajer V, Schmidt M, Pahl R, Moffat K, Structure. 2005 Jan;13(1):55-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15642261 15642261]
</table>
== Function ==
[https://www.uniprot.org/uniprot/PYP_HALHA PYP_HALHA] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t1/1t1b_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t1b ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Halorhodospira halophila]]
[[Category: Halorhodospira halophila]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Anderson, S.]]
[[Category: Anderson S]]
[[Category: Moffat, K.]]
[[Category: Moffat K]]
[[Category: Pahl, R.]]
[[Category: Pahl R]]
[[Category: Rajagopal, S.]]
[[Category: Rajagopal S]]
[[Category: Schmidt, M.]]
[[Category: Schmidt M]]
[[Category: Srajer, V.]]
[[Category: Srajer V]]
[[Category: HC4]]
[[Category: pas domain]]
[[Category: photoactive yellow protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:55:03 2007''

Latest revision as of 11:37, 1 May 2024

Late intermediate IL2 from time-resolved crystallography of the E46Q mutant of PYPLate intermediate IL2 from time-resolved crystallography of the E46Q mutant of PYP

Structural highlights

1t1b is a 1 chain structure with sequence from Halorhodospira halophila. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.6Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PYP_HALHA Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1t1b, resolution 1.60Å

Drag the structure with the mouse to rotate

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