1sv0: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1sv0.jpg|left|200px]]<br /><applet load="1sv0" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1sv0, resolution 2.07&Aring;" />
-'''Crystal Structure Of Yan-SAM/Mae-SAM Complex'''<br />
-==Overview==
+==Crystal Structure Of Yan-SAM/Mae-SAM Complex==
-Yan, an ETS family transcriptional repressor, is regulated by receptor, tyrosine kinase signaling via the Ras/MAPK pathway. Phosphorylation and, downregulation of Yan is facilitated by a protein called Mae. Yan and Mae, interact through their SAM domains. We find that repression by Yan, requires the formation of a higher order structure mediated by Yan-SAM, polymerization. Moreover, a crystal structure of the Yan-SAM/Mae-SAM, complex shows that Mae-SAM specifically recognizes a surface on Yan-SAM, that is also required for Yan-SAM polymerization. Mae-SAM binds to Yan-SAM, with approximately 1000-fold higher affinity than Yan-SAM binds to itself, and can effectively depolymerize Yan-SAM. Mutations on Mae that, specifically disrupt its SAM domain-dependent interactions with Yan, disable the derepression function of Mae in vivo. Depolymerization of Yan, by Mae represents a novel mechanism of transcriptional control that, sensitizes Yan for regulation by receptor tyrosine kinases.
+<StructureSection load='1sv0' size='340' side='right'caption='[[1sv0]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1sv0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SV0 FirstGlance]. <br>
-SV0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SV0 OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sv0 OCA], [https://pdbe.org/1sv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sv0 RCSB], [https://www.ebi.ac.uk/pdbsum/1sv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sv0 ProSAT]</span></td></tr>
-==Reference==
+</table>
-Derepression by depolymerization; structural insights into the regulation of Yan by Mae., Qiao F, Song H, Kim CA, Sawaya MR, Hunter JB, Gingery M, Rebay I, Courey AJ, Bowie JU, Cell. 2004 Jul 23;118(2):163-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15260987 15260987]
+== Function ==
+[https://www.uniprot.org/uniprot/POK_DROME POK_DROME] Negative regulator of photoreceptor development that acts antagonistically to the proneural signal mediated by RAS. It acts upstream of SINA to inhibit R7 development.<ref>PMID:1505027</ref> <ref>PMID:1495974</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sv/1sv0_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sv0 ConSurf].
+<div style="clear:both"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Drosophila melanogaster]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Bowie, J.U.]]
+[[Category: Bowie JU]]
-[[Category: Courey, A.J.]]
+[[Category: Courey AJ]]
-[[Category: Gingery, M.]]
+[[Category: Gingery M]]
-[[Category: Hunter, J.B.]]
+[[Category: Hunter JB]]
-[[Category: Kim, C.A.]]
+[[Category: Kim CA]]
-[[Category: Qiao, F.]]
+[[Category: Qiao F]]
-[[Category: Rebay, I.]]
+[[Category: Rebay I]]
-[[Category: Sawaya, M.R.]]
+[[Category: Sawaya MR]]
-[[Category: Song, H.]]
+[[Category: Song H]]
-[[Category: 3(10) helix]]
-[[Category: alpha-helix]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:41:53 2007''