3d0q: Difference between revisions

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-{{Seed}}
-[[Image:3d0q.png|left|200px]]
-<!--
+==Crystal structure of calG3 from Micromonospora echinospora determined in space group I222==
-The line below this paragraph, containing "STRUCTURE_3d0q", creates the "Structure Box" on the page.
+<StructureSection load='3d0q' size='340' side='right'caption='[[3d0q]], [[Resolution|resolution]] 2.79&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[3d0q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D0Q FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.79&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-{{STRUCTURE_3d0q|  PDB=3d0q  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d0q OCA], [https://pdbe.org/3d0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d0q RCSB], [https://www.ebi.ac.uk/pdbsum/3d0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d0q ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8KND7_MICEC Q8KND7_MICEC]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d0/3d0q_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3d0q ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The enediyne antibiotic calicheamicin (CLM) gamma(1)(I) is a prominent antitumor agent that is targeted to DNA by a novel aryltetrasaccharide comprised of an aromatic unit and four unusual carbohydrates. Herein we report the heterologous expression and the biochemical characterization of the two "internal" glycosyltransferases CalG3 and CalG2 and the structural elucidation of an enediyne glycosyltransferase (CalG3). In conjunction with the previous characterization of the "external" CLM GTs CalG1 and CalG4, this study completes the functional assignment of all four CLM GTs, extends the utility of enediyne GT-catalyzed reaction reversibility, and presents conclusive evidence of a sequential glycosylation pathway in CLM biosynthesis. This work also reveals the common GT-B structural fold can now be extended to include enediyne GTs.
-===Crystal structure of calG3 from Micromonospora echinospora determined in space group I222===
+Biochemical and structural insights of the early glycosylation steps in calicheamicin biosynthesis.,Zhang C, Bitto E, Goff RD, Singh S, Bingman CA, Griffith BR, Albermann C, Phillips GN Jr, Thorson JS Chem Biol. 2008 Aug 25;15(8):842-53. PMID:18721755<ref>PMID:18721755</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_18721755}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 3d0q" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 18721755 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_18721755}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-D0Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D0Q OCA].
-==Reference==
-Biochemical and structural insights of the early glycosylation steps in calicheamicin biosynthesis., Zhang C, Bitto E, Goff RD, Singh S, Bingman CA, Griffith BR, Albermann C, Phillips GN Jr, Thorson JS, Chem Biol. 2008 Aug;15(8):842-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18721755 18721755]
 [[Category: Micromonospora echinospora]]
-[[Category: Single protein]]
+[[Category: Bingman CA]]
-[[Category: Bingman, C A.]]
+[[Category: Bitto E]]
-[[Category: Bitto, E.]]
+[[Category: Phillips Jr GN]]
-[[Category: Jr., G N.Phillips.]]
+[[Category: Singh S]]
-[[Category: Singh, S.]]
+[[Category: Wesenberg GE]]
-[[Category: Wesenberg, G E.]]
-[[Category: Calicheamicin synthesis]]
-[[Category: Enediyne antibiotic]]
-[[Category: Glycosyltransferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 10 08:55:15 2008''