2vyw: Difference between revisions
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< | ==Hemoglobin (Hb2) from trematode Fasciola hepatica== | ||
<StructureSection load='2vyw' size='340' side='right'caption='[[2vyw]], [[Resolution|resolution]] 1.80Å' scene=''> | |||
You may | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2vyw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fasciola_hepatica Fasciola hepatica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VYW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VYW FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | |||
-- | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vyw OCA], [https://pdbe.org/2vyw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vyw RCSB], [https://www.ebi.ac.uk/pdbsum/2vyw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vyw ProSAT]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vy/2vyw_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vyw ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The trematode Fasciola hepatica (Fa.he.) is a common parasite of human and livestock. The hemoglobin (Hb) of Fa.he., a potential immunogen, was chosen for characterization in the search for an effective vaccine. Characterization of trematode Hbs show that they are intracellular single-domain globins with the following remarkable features: (1) Fa.he. expresses two Hb isoforms that differ at two amino acid sites (F1: 119Y/123Q; F2: 119F/123L). Both isoforms are monoacetylated at their N-termini; (2) the genes coding for Fa.he. and Paramphistomum epiclitum (Pa.ep.) Hbs are interrupted by two introns at the conserved positions B12.2 and G7.0.; (3) UV/VIS and resonance Raman spectroscopy identify the recombinant Fa.he. HbF2 as a pentacoordinated high-spin ferrous Hb; (4) electron paramagnetic resonance spectroscopy of cyano-met Fa.he. HbF2 proves that the endogenously bound imidazole has no imidazolate character; (5) the major structural determinants of the globin fold are present, they contain a TyrB10/TyrE7 residue pair on the distal side. Although such distal-site pair is a signature for high oxygen affinity, as shown for Pa.ep. Hb, the oxygen-binding rate parameters for Fa.he. Hb are intermediate between those of myoglobin and those of other trematode Hbs; (6) the three-dimensional structure of recombinant Fa.he. HbF2 from this study closely resembles the three-dimensional structure of Pa.ep. determined earlier. The set of distal-site polar interactions observed in Pa.ep. Hb is matched with small but significant structural adjustments; (7) despite the potential immunogenic character of the fluke Hb, vaccination of calves with recombinant Fa.he. HbF2 failed to promote protection against parasitic infection. | |||
The hemoglobins of the trematodes Fasciola hepatica and Paramphistomum epiclitum: a molecular biological, physico-chemical, kinetic, and vaccination study.,Dewilde S, Ioanitescu AI, Kiger L, Gilany K, Marden MC, Van Doorslaer S, Vercruysse J, Pesce A, Nardini M, Bolognesi M, Moens L Protein Sci. 2008 Oct;17(10):1653-62. Epub 2008 Jul 11. PMID:18621914<ref>PMID:18621914</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2vyw" style="background-color:#fffaf0;"></div> | |||
== | ==See Also== | ||
*[[Hemoglobin 3D structures|Hemoglobin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Fasciola hepatica]] | [[Category: Fasciola hepatica]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Bolognesi | [[Category: Bolognesi M]] | ||
[[Category: Dewilde | [[Category: Dewilde S]] | ||
[[Category: Gilany K]] | |||
[[Category: Gilany | [[Category: Ioanitescu AI]] | ||
[[Category: Ioanitescu | [[Category: Kiger L]] | ||
[[Category: Kiger | [[Category: Marden MC]] | ||
[[Category: Marden | [[Category: Moens L]] | ||
[[Category: Moens | [[Category: Nardini M]] | ||
[[Category: Nardini | [[Category: Pesce A]] | ||
[[Category: Pesce | [[Category: Van Doorslaer S]] | ||
[[Category: | [[Category: Vercruysse J]] | ||
[[Category: | |||