1qxp: Difference between revisions

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New page: left|200px<br /><applet load="1qxp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qxp, resolution 2.80Å" /> '''Crystal Structure of...
 
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'''Crystal Structure of a mu-like calpain'''<br />


==Overview==
==Crystal Structure of a mu-like calpain==
The two Ca2+-dependent cysteine proteases, micro- and m-calpain, are, involved in various Ca2+-linked signal pathways but differ markedly in, their Ca2+ requirements for activation. We have determined the structure, of a micro-like calpain, which has 85% micro-calpain sequence (the first, 48 and the last 62 residues of the large subunit are those from m-calpain), and a low Ca2+ requirement. This construct was used because micro-calpain, itself is too poorly expressed. The structure of micro-like calpain is, very similar in overall fold to that of m-calpain as expected, but differs, significantly in two aspects. In comparison with m-calpain, the catalytic, triad residues in micro-like calpain, His and Cys, are much closer, together in the absence of Ca2+, and significant portions of the Ca2+, binding EF-hand motifs are disordered and more flexible. These structural, differences imply that Ca2+-free micro-calpain may represent a partially, activated structure, requiring lower Ca2+ concentration to trigger its, activation.
<StructureSection load='1qxp' size='340' side='right'caption='[[1qxp]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1qxp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QXP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxp OCA], [https://pdbe.org/1qxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qxp RCSB], [https://www.ebi.ac.uk/pdbsum/1qxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qxp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CPNS1_RAT CPNS1_RAT] Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.[https://www.uniprot.org/uniprot/CAN1_RAT CAN1_RAT] Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.[https://www.uniprot.org/uniprot/CAN2_RAT CAN2_RAT] Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qx/1qxp_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qxp ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1QXP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Active as [http://en.wikipedia.org/wiki/Calpain-1 Calpain-1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.52 3.4.22.52] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QXP OCA].
*[[Calpain 3D structures|Calpain 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of a micro-like calpain reveals a partially activated conformation with low Ca2+ requirement., Pal GP, De Veyra T, Elce JS, Jia Z, Structure. 2003 Dec;11(12):1521-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14656436 14656436]
[[Category: Bos taurus]]
[[Category: Calpain-1]]
[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Single protein]]
[[Category: Elce JS]]
[[Category: Elce, J.S.]]
[[Category: Jia Z]]
[[Category: Jia, Z.]]
[[Category: Pal GP]]
[[Category: Pal, G.P.]]
[[Category: Veyra TD]]
[[Category: Veyra, T.D.]]
[[Category: ca(2+) requirement]]
[[Category: catalytic triad]]
[[Category: m-calpain]]
[[Category: mu-calpain]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:05:58 2007''

Latest revision as of 11:18, 14 February 2024

Crystal Structure of a mu-like calpainCrystal Structure of a mu-like calpain

Structural highlights

1qxp is a 2 chain structure with sequence from Bos taurus and Rattus norvegicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.8Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CPNS1_RAT Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.CAN1_RAT Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.CAN2_RAT Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1qxp, resolution 2.80Å

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OCA
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