2je2: Difference between revisions

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-{{Seed}}
-[[Image:2je2.png|left|200px]]
-<!--
+==Cytochrome P460 from Nitrosomonas europaea - probable nonphysiological oxidized form==
-The line below this paragraph, containing "STRUCTURE_2je2", creates the "Structure Box" on the page.
+<StructureSection load='2je2' size='340' side='right'caption='[[2je2]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2je2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitrosomonas_europaea Nitrosomonas europaea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JE2 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-{{STRUCTURE_2je2|  PDB=2je2  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2je2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2je2 OCA], [https://pdbe.org/2je2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2je2 RCSB], [https://www.ebi.ac.uk/pdbsum/2je2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2je2 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q50927_NITER Q50927_NITER]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/je/2je2_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2je2 ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We have determined the 1.8 A X-ray crystal structure of a monoheme c-type cytochrome, cytochrome P460, from Nitrosomonas europea. The chromophore possesses unusual spectral properties analogous to those of the catalytic heme P460 of hydroxylamine oxidoreductase (HAO), the only known heme in biology to withdraw electrons from an iron-coordinated substrate. The analysis reveals a homodimeric structure and elucidates a new c-type cytochrome fold that is predominantly beta-sheet. In addition to the two cysteine thioether links to the porphyrin typical of c-type hemes, there is a third proteinaceous link involving a conserved lysine. The covalent bond is between the lysine side-chain nitrogen and the 13'-meso carbon of the heme, which, following cross-link formation, is sp3-hybridized, demonstrating the loss of conjugation at this position within the porphyrin. The structure has implications for the analogous tyrosine-heme meso carbon cross-link observed in HAO.
-===CYTOCHROME P460 FROM NITROSOMONAS EUROPAEA- PROBABLE NONPHYSIOLOGICAL OXIDIZED FORM===
+The crystal structure of cytochrome P460 of Nitrosomonas europaea reveals a novel cytochrome fold and heme-protein cross-link.,Pearson AR, Elmore BO, Yang C, Ferrara JD, Hooper AB, Wilmot CM Biochemistry. 2007 Jul 17;46(28):8340-9. Epub 2007 Jun 21. PMID:17583915<ref>PMID:17583915</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_17583915}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 2je2" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 17583915 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_17583915}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-JE2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nitrosomonas_europaea Nitrosomonas europaea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE2 OCA].
-==Reference==
-The crystal structure of cytochrome P460 of Nitrosomonas europaea reveals a novel cytochrome fold and heme-protein cross-link., Pearson AR, Elmore BO, Yang C, Ferrara JD, Hooper AB, Wilmot CM, Biochemistry. 2007 Jul 17;46(28):8340-9. Epub 2007 Jun 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17583915 17583915]
 [[Category: Nitrosomonas europaea]]
-[[Category: Single protein]]
+[[Category: Elmore BO]]
-[[Category: Elmore, B O.]]
+[[Category: Ferrara JD]]
-[[Category: Ferrara, J D.]]
+[[Category: Hooper AB]]
-[[Category: Hooper, A B.]]
+[[Category: Pearson AR]]
-[[Category: Pearson, A R.]]
+[[Category: Wilmot CM]]
-[[Category: Wilmot, C M.]]
+[[Category: Yang C]]
-[[Category: Yang, C.]]
-[[Category: Cross-linked heme]]
-[[Category: Cytochrome p460]]
-[[Category: Heme p460]]
-[[Category: Metal binding protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:56:45 2008''