1q13: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1q13.gif|left|200px]]<br /><applet load="1q13" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1q13, resolution 2.08&Aring;" />
-'''Crystal structure of rabbit 20alpha hyroxysteroid dehydrogenase in ternary complex with NADP and testosterone'''<br />
-==Overview==
+==Crystal structure of rabbit 20alpha hyroxysteroid dehydrogenase in ternary complex with NADP and testosterone==
-The aldo-keto reductase rabbit 20alpha-hydroxysteroid dehydrogenase, (rb20alpha-HSD; AKR1C5) is less selective than other HSDs, since it exerts, its activity both on androgens (C19 steroids) and progestins (C21, steroids). In order to identify the molecular determinants responsible for, this reduced selectivity, binary (NADPH) and ternary, (NADP(+)/testosterone) complex structures were solved to 1.32A and 2.08A, resolution, respectively. Inspection of the cofactor-binding cavity led to, the identification of a new interaction between side-chains of residues, His222 and Lys270, which cover the central phosphate chain of the, cofactor, reminiscent of the "safety-belt" found in other aldo-keto, reductases. Testosterone is stabilized by a phenol/benzene tunnel composed, of side-chains of numerous residues, among which Phe54, which forces the, steroid to take up an orientation markedly contrasting with that found in, HSD ternary complexes reported. Combining structural, site-directed, mutagenesis, kinetic and fluorescence titration studies, we found that the, selectivity of rb20alpha-HSD is mediated by (i) the relaxation of loop B, (residues 223-230), partly controlled by the nature of residue 230, (ii), the nature of the residue found at position 54, and (iii) the residues, found in the C-terminal tail of the protein especially the side-chain of, the amino acid 306.
+<StructureSection load='1q13' size='340' side='right'caption='[[1q13]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1q13]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q13 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q13 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.08&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TES:TESTOSTERONE'>TES</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q13 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q13 OCA], [https://pdbe.org/1q13 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q13 RCSB], [https://www.ebi.ac.uk/pdbsum/1q13 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q13 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PE2R_RABIT PE2R_RABIT] Can convert prostaglandin E2 to prostaglandin F2-alpha.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q1/1q13_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q13 ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-Q13 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with SO4, NAP and TES as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q13 OCA].
+*[[Hydroxysteroid dehydrogenase 3D structures|Hydroxysteroid dehydrogenase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Loop relaxation, a mechanism that explains the reduced specificity of rabbit 20alpha-hydroxysteroid dehydrogenase, a member of the aldo-keto reductase superfamily., Couture JF, Legrand P, Cantin L, Labrie F, Luu-The V, Breton R, J Mol Biol. 2004 May 21;339(1):89-102. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15123423 15123423]
+[[Category: Large Structures]]
 [[Category: Oryctolagus cuniculus]]
-[[Category: Single protein]]
+[[Category: Breton R]]
-[[Category: Breton, R.]]
+[[Category: Cantin L]]
-[[Category: Cantin, L.]]
+[[Category: Couture J-F]]
-[[Category: Couture, J.F.]]
+[[Category: Labrie F]]
-[[Category: Labrie, F.]]
+[[Category: Legrand P]]
-[[Category: Legrand, P.]]
+[[Category: Luu-The V]]
-[[Category: Luu-The, V.]]
-[[Category: NAP]]
-[[Category: SO4]]
-[[Category: TES]]
-[[Category: 20alpha-hsd]]
-[[Category: akr]]
-[[Category: aldo-keto reductase]]
-[[Category: hydroxysteroid dehydrogenase]]
-[[Category: ternary complex]]
-[[Category: testosterone]]
-[[Category: tim-barrel]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:17:41 2007''