1stx: Difference between revisions

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{{Seed}}
[[Image:1stx.png|left|200px]]


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==Structure of the K38A mutant of EcoRV bound to cognate DNA and Mn2+==
The line below this paragraph, containing "STRUCTURE_1stx", creates the "Structure Box" on the page.
<StructureSection load='1stx' size='340' side='right'caption='[[1stx]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1stx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1STX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1STX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
{{STRUCTURE_1stx|  PDB=1stx  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1stx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1stx OCA], [https://pdbe.org/1stx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1stx RCSB], [https://www.ebi.ac.uk/pdbsum/1stx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1stx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/T2E5_ECOLX T2E5_ECOLX] Recognizes the double-stranded sequence GATATC and cleaves after T-3.


===Structure of the K38A mutant of EcoRV bound to cognate DNA and Mn2+===
==See Also==
 
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 
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{{ABSTRACT_PUBMED_15170321}}
 
==About this Structure==
1STX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1STX OCA].
 
==Reference==
DNA cleavage by EcoRV endonuclease: two metal ions in three metal ion binding sites., Horton NC, Perona JJ, Biochemistry. 2004 Jun 8;43(22):6841-57. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15170321 15170321]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Horton NC]]
[[Category: Horton, N C.]]
[[Category: Perona JJ]]
[[Category: Perona, J J.]]
[[Category: Dna]]
[[Category: Ecorv]]
[[Category: Restriction enzyme]]
[[Category: X-ray crystallography]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 04:57:06 2008''

Latest revision as of 11:33, 14 February 2024

Structure of the K38A mutant of EcoRV bound to cognate DNA and Mn2+Structure of the K38A mutant of EcoRV bound to cognate DNA and Mn2+

Structural highlights

1stx is a 6 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

T2E5_ECOLX Recognizes the double-stranded sequence GATATC and cleaves after T-3.

See Also

1stx, resolution 2.10Å

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