1opf: Difference between revisions

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OCA

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-[[Image:1opf.jpg|left|200px]]<br /><applet load="1opf" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1opf, resolution 3.2&Aring;" />
-'''THE STRUCTURE OF OMPF PORIN IN A TETRAGONAL CRYSTAL FORM'''<br />
-==Overview==
+==THE STRUCTURE OF OMPF PORIN IN A TETRAGONAL CRYSTAL FORM==
-BACKGROUND: OmpF porin is a trimeric integral membrane protein responsible, for the passive transport of small hydrophilic molecules, such as, nutrients and waste products, across the outer membrane of Escherichia, coli. Very few membrane proteins have been crystallized in three, dimensions, yet this stable protein can be obtained in several crystal, forms. Comparison of the structures of the same membrane protein in two, different packing environments is of major interest, because it allows us, to explore the integrity of the structure outside the natural membrane, environment. RESULTS: The structure of OmpF porin in a tetragonal crystal, form with two trimers per asymmetric unit has been determined at 3.2 A, resolution and compared with that obtained previously in a trigonal, crystal form. The lattice contacts involve only polar atoms, whereas, extensive hydrophobic protein-protein interactions were found in the, trigonal lattice. The trimer structure is virtually identical in both., CONCLUSIONS: Our comparison reveals that the overall structure of OmpF is, not influenced by crystal lattice constraints and, thus, presumably bears, close resemblance to the in vivo structure. The tetragonal crystal, structure has provided the starting model for the phasing of neutron, diffraction data obtained from this crystal form, as described in an, accompanying article.
+<StructureSection load='1opf' size='340' side='right'caption='[[1opf]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1opf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OPF FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1opf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1opf OCA], [https://pdbe.org/1opf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1opf RCSB], [https://www.ebi.ac.uk/pdbsum/1opf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1opf ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/OMPF_ECOLI OMPF_ECOLI] Forms pores that allow passive diffusion of small molecules across the outer membrane. It is also a receptor for the bacteriophage T2.<ref>PMID:19721064</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/op/1opf_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1opf ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-OPF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OPF OCA].
+*[[Porin 3D structures|Porin 3D structures]]
+== References ==
-==Reference==
+<references/>
-The structure of OmpF porin in a tetragonal crystal form., Cowan SW, Garavito RM, Jansonius JN, Jenkins JA, Karlsson R, Konig N, Pai EF, Pauptit RA, Rizkallah PJ, Rosenbusch JP, et al., Structure. 1995 Oct 15;3(10):1041-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8589999 8589999]
+__TOC__
+</StructureSection>
 [[Category: Escherichia coli]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Cowan, S.W.]]
+[[Category: Cowan SW]]
-[[Category: Jansonius, J.N.]]
+[[Category: Jansonius JN]]
-[[Category: Pauptit, R.A.]]
+[[Category: Pauptit RA]]
-[[Category: Schirmer, T.]]
+[[Category: Schirmer T]]
-[[Category: membrane protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:05:11 2007''