1gvy: Difference between revisions

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New page: left|200px<br /> <applet load="1gvy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gvy, resolution 1.70Å" /> '''SUBSTRATE DISTORSIO...
 
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[[Image:1gvy.gif|left|200px]]<br />
<applet load="1gvy" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1gvy, resolution 1.70&Aring;" />
'''SUBSTRATE DISTORSION BY BETA-MANNANASE FROM PSEUDOMONAS CELLULOSA'''<br />


==About this Structure==
==Substrate distorsion by beta-mannanase from Pseudomonas cellulosa==
1GVY is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_cellulosa Pseudomonas cellulosa]] with ZN, NA, NIN, MBF and TRS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GVY OCA]].  
<StructureSection load='1gvy' size='340' side='right'caption='[[1gvy]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1gvy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GVY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GVY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MBF:2-DEOXY-2-FLUORO-BETA-D-MANNOSE'>MBF</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NIN:DINITROPHENYLENE'>NIN</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gvy OCA], [https://pdbe.org/1gvy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gvy RCSB], [https://www.ebi.ac.uk/pdbsum/1gvy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gvy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MANA_CELJU MANA_CELJU] Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. Preferentially hydrolyzes the larger oligosaccharides and has greater activity against non-substituted polysaccharides.<ref>PMID:7848261</ref> <ref>PMID:11064195</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gv/1gvy_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gvy ConSurf].
<div style="clear:both"></div>


==Reference==
==See Also==
Substrate distortion by a beta-mannanase: snapshots of the Michaelis and covalent-intermediate complexes suggest a B(2,5) conformation for the transition state., Ducros VM, Zechel DL, Murshudov GN, Gilbert HJ, Szabo L, Stoll D, Withers SG, Davies GJ, Angew Chem Int Ed Engl. 2002 Aug 2;41(15):2824-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12203498 12203498]
*[[Mannosidase 3D structures|Mannosidase 3D structures]]
[[Category: Pseudomonas cellulosa]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Davies, G.J.]]
__TOC__
[[Category: Ducros, V.]]
</StructureSection>
[[Category: Gilbert, H.J.]]
[[Category: Cellvibrio japonicus]]
[[Category: Stoll, D.]]
[[Category: Large Structures]]
[[Category: Szabo, L.]]
[[Category: Davies GJ]]
[[Category: Withers, S.G.]]
[[Category: Ducros V]]
[[Category: Zechel, D.L.]]
[[Category: Gilbert HJ]]
[[Category: MBF]]
[[Category: Szabo L]]
[[Category: NA]]
[[Category: Withers SG]]
[[Category: NIN]]
[[Category: Zechel DL]]
[[Category: TRS]]
[[Category: ZN]]
[[Category: family 26]]
[[Category: glycosidase]]
[[Category: glycoside hydrolase]]
[[Category: mannan]]
[[Category: mannanase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 20:38:50 2007''

Latest revision as of 15:09, 13 December 2023

Substrate distorsion by beta-mannanase from Pseudomonas cellulosaSubstrate distorsion by beta-mannanase from Pseudomonas cellulosa

Structural highlights

1gvy is a 1 chain structure with sequence from Cellvibrio japonicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.7Å
Ligands:, , , , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MANA_CELJU Hydrolyzes mannan and galactomannan, but displays little activity towards other polysaccharides located in the plant cell wall. Appears to act in synergy with alpha-galactosidase (AgaA) to elicit hydrolysis of galactomannan. Preferentially hydrolyzes the larger oligosaccharides and has greater activity against non-substituted polysaccharides.[1] [2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Braithwaite KL, Black GW, Hazlewood GP, Ali BR, Gilbert HJ. A non-modular endo-beta-1,4-mannanase from Pseudomonas fluorescens subspecies cellulosa. Biochem J. 1995 Feb 1;305 ( Pt 3):1005-10. PMID:7848261
  2. ↑ Halstead JR, Fransen MP, Eberhart RY, Park AJ, Gilbert HJ, Hazlewood GP. alpha-Galactosidase A from Pseudomonas fluorescens subsp. cellulosa: cloning, high level expression and its role in galactomannan hydrolysis. FEMS Microbiol Lett. 2000 Nov 15;192(2):197-203. PMID:11064195

1gvy, resolution 1.70Å

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