1nx0: Difference between revisions

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New page: left|200px<br /><applet load="1nx0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nx0, resolution 2.3Å" /> '''Structure of Calpain ...
 
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[[Image:1nx0.jpg|left|200px]]<br /><applet load="1nx0" size="450" color="white" frame="true" align="right" spinBox="true"
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'''Structure of Calpain Domain 6 in Complex with Calpastatin DIC'''<br />


==Overview==
==Structure of Calpain Domain 6 in Complex with Calpastatin DIC==
The Ca(2+)-dependent cysteine protease calpain along with its endogenous, inhibitor calpastatin is widely distributed. The interactions between, calpain and calpastatin have been studied to better understand the nature, of calpain inhibition by calpastatin, which can aid the design of small, molecule inhibitors to calpain. Here we present the crystal structure of a, complex between a calpastatin peptide and the calcium-binding domain VI of, calpain. DIC19 is a 19 residue peptide, which corresponds to one of the, three interacting domains of calpastatin, which is known to interact with, domain VI of calpain. We present two crystal structures of DIC19 bound to, domain VI of calpain, determined by molecular replacement methods to 2.5A, and 2.2A resolution. In the process of crystallizing the inhibitor, complex, a new native crystal form was identified which had the homodimer, 2-fold axis along a crystallographic axis as opposed to the previously, observed dimer in the asymmetric unit. The crystal structures of the, native domain VI and its inhibitor PD150606, (3-(4-iodophenyl)-2-mercapto-(Z)-2-propenoic acid) complex were determined, with the help of molecular replacement methods to 2.0A and 2.3A, resolution, respectively. In addition, we built a homology model for the, complex between domain IV and DIA19 peptide of calpastatin. Finally, we, present a model for the calpastatin-inhibited calpain.
<StructureSection load='1nx0' size='340' side='right'caption='[[1nx0]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1nx0]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NX0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nx0 OCA], [https://pdbe.org/1nx0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nx0 RCSB], [https://www.ebi.ac.uk/pdbsum/1nx0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nx0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CPNS1_PIG CPNS1_PIG] Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nx/1nx0_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nx0 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1NX0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.52 and 3.4.22.53 3.4.22.52 and 3.4.22.53] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NX0 OCA].
*[[Calpain 3D structures|Calpain 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
A structural model for the inhibition of calpain by calpastatin: crystal structures of the native domain VI of calpain and its complexes with calpastatin peptide and a small molecule inhibitor., Todd B, Moore D, Deivanayagam CC, Lin GD, Chattopadhyay D, Maki M, Wang KK, Narayana SV, J Mol Biol. 2003 Apr 18;328(1):131-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12684003 12684003]
[[Category: Large Structures]]
[[Category: Hydrolase]]
[[Category: Protein complex]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
[[Category: Chattopadhyay, D.]]
[[Category: Chattopadhyay D]]
[[Category: Deivanayagam, C.C.S.]]
[[Category: Deivanayagam CCS]]
[[Category: Lin, G.D.]]
[[Category: Lin G-D]]
[[Category: Maki, M.]]
[[Category: Maki M]]
[[Category: Moore, D.]]
[[Category: Moore D]]
[[Category: Narayana, S.V.L.]]
[[Category: Narayana SVL]]
[[Category: Todd, B.]]
[[Category: Todd B]]
[[Category: Wang, K.K.W.]]
[[Category: Wang KKW]]
[[Category: CA]]
[[Category: calcium binding]]
[[Category: hydrolase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 22:37:57 2007''

Latest revision as of 11:00, 14 February 2024

Structure of Calpain Domain 6 in Complex with Calpastatin DICStructure of Calpain Domain 6 in Complex with Calpastatin DIC

Structural highlights

1nx0 is a 5 chain structure with sequence from Sus scrofa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.3Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CPNS1_PIG Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1nx0, resolution 2.30Å

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