1chk: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1chk.gif|left|200px]]<br />
-<applet load="1chk" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1chk, resolution 2.40&Aring;" />
-'''STREPTOMYCES N174 CHITOSANASE PH5.5 298K'''<br />
-==Overview==
+==STREPTOMYCES N174 CHITOSANASE PH5.5 298K==
-We report the 2.4 A X-ray crystal structure of a protein with chitosan, endo-hydrolase activity isolated from Streptomyces N174. The structure was, solved using phases acquired by SIRAS from a two-site methyl mercury, derivative combined with solvent flattening and non-crystallographic, two-fold symmetry averaging, and refined to an R-factor of 18.5%. The, mostly alpha-helical fold reveals a structural core shared with several, classes of lysozyme and barley endochitinase, in spite of a lack of shared, sequence. Based on this structural similarity we postulate a putative, active site, mechanism of action and mode of substrate recognition. It, appears that Glu 22 acts as an acid and Asp 40 serves as a general base to, activate a water molecule for an SN2 attack on the glycosidic bond. A, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?8564542 (full description)]]
+<StructureSection load='1chk' size='340' side='right'caption='[[1chk]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1chk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._N174 Streptomyces sp. N174]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CHK FirstGlance]. <br>
-CHK is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CHK OCA]].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1chk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1chk OCA], [https://pdbe.org/1chk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1chk RCSB], [https://www.ebi.ac.uk/pdbsum/1chk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1chk ProSAT]</span></td></tr>
-==Reference==
+</table>
-X-ray structure of an anti-fungal chitosanase from streptomyces N174., Marcotte EM, Monzingo AF, Ernst SR, Brzezinski R, Robertus JD, Nat Struct Biol. 1996 Feb;3(2):155-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8564542 8564542]
+== Function ==
-[[Category: Single protein]]
+[https://www.uniprot.org/uniprot/CHIS_STRSN CHIS_STRSN] Aids in the defense against invading fungal pathogens by degrading their cell wall chitosan.
-[[Category: Streptomyces sp.]]
+== Evolutionary Conservation ==
-[[Category: Marcotte, E.M.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Robertus, J.D.]]
+Check<jmol>
-[[Category: anti-fungal protein]]
+  <jmolCheckbox>
-[[Category: hydrolase]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ch/1chk_consurf.spt"</scriptWhenChecked>
-[[Category: o-glycosyl]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 20:35:31 2007''
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1chk ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Streptomyces sp. N174]]
+[[Category: Marcotte EM]]
+[[Category: Robertus JD]]